Cell Signaling(CST) 13788 PathScan® Immune Cell Signaling Antibody Array Kit (Fluorescent Readout)

产品编号 13788 | Cell Signaling(CST)

产品描述

Kit Includes	Quantity	Applications	Reactivity	Homology†	MW (kDa)	Isotype
Inflammation-Immune Antibody Array 10X Detection Cocktail	300 µl
Immune Cell Signaling Antibody Array Slides	2 ea				0
16-Well Gasket	2 ea
PathScan^® Sandwich ELISA Lysis Buffer (1X) #7018	30 ml
20X Array Wash Buffer	15 ml
DyLight 680TM-linked Streptavidin (10X)	300 µl
Sealing Tape	2 sheets
Array Blocking Buffer	5 ml
Array Diluent Buffer	15 ml

Specificity / Sensitivity

PathScan^® Immune Cell Signaling Antibody Array Kit (Fluorescent Readout) detects the target proteins as specified on the Array Target Map. No substantial cross-reactivity has been observed between targets. This kit is optimized for cell lysates diluted to a total protein concentration between 0.2 and 1 mg/ml (see kit protocol). All sandwich assays have been validated for human derived samples. This kit detects proteins from the indicated species as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan^® Immune Cell Signaling Antibody Array Kit (Fluorescent Readout) uses glass slides as the planar surface and is based upon the sandwich immunoassay principle. The array kit allows for the simultaneous detection of 19 signaling molecules that are involved in the regulation of the immune and the inflammatory responses. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two slides allowing for the interrogation of 32 different samples and the generation of 608 data points in a single experiment. Cell lysates are incubated on the slide followed by a biotinylated detection antibody cocktail. DyLight™ 680-linked Streptavidin is then used to visualize the bound detection antibody. A fluorescent image of the slide can then be captured with a digital imaging system and spot intensities quantified using array analysis software.

Background

The vertebrate immune response relies on both a non-specific, innate immune system and an antigen-specific, adaptive immune response. Organisms rely on cell-mediated and antibody-mediated responses to detect pathogens and protect against infection and disease. Inflammation is an immune response in which circulating immune system cells localize to sites of injury or infection. Regulation of inflammation is essential, as unchecked inflammation can result in permanent damage to tissues and organs. A variety of signaling proteins are known to regulate the immune and inflammatory response.
Akt and p44/42 MAPK (Erk1/2) kinases are key regulators of cell fate that generally transmit growth promoting and survival signals. These two kinases serve as major signal integration hubs and very few cellular processes occur without their involvement. Both p38 MAPK and SAPK/JNK kinases are activated through a dual phosphorylation mechanism in response to pro-inflammatory cytokines, stressful conditions, or genotoxic stress. Caspase-7 is an intracellular protease involved in apoptosis and is part of a cellular sub-organelle called the inflammasome. Caspase-7 is activated by cleavage at Asp198. Activation of the essential immune system regulator NF-κB is triggered by a diverse group of extracellular signals promoted by inflammatory cytokines, growth factors, and chemokines. The proteasome-mediated degradation of the NF-κB/Rel inhibitor IκBα results from phosphorylation of IκBα at Ser32 and Ser36 and targeting of IκBα to the proteasome. The TAK1 kinase responds to a variety of cytokines to regulate cellular kinases and activate the NF-κB pathway; phosphorylation of TAK1 at Ser412 by PKA regulates kinase activity.
Stat family transcription factors are activated by a variety of cytokines, chemokines, or growth factors to regulate the immune response. Phosphorylation of Stat proteins at specific tyrosine or serine residues leads to Stat protein dimerization, nuclear translocation, and binding of the transcription factor to genes that regulate the immune response. The range and specificity of responses regulated by Stat proteins is determined in part by the tissue-specific expression of different cytokine receptors and by the combinatorial coupling of various Stat members to different receptors. Lck, Syk, and Zap-70 are Src family tyrosine kinases that couple the activated B cells and T cell immunoreceptors to downstream signaling events that mediate the immune response. Rig-1 is a Toll-receptor signaling complex component that plays a role in the antiviral innate immune response. The transcription factor IRF-3 plays a role in regulating interferon (IFN) and IFN-inducible gene expression in response to viral infection.

常见问题及解答

公司介绍

CST公司是一家由研究型科学家创建于1999年的家族式私营企业，在全球范围内拥有超过400多名员工。CST活跃于应用系统生物学研究，尤其是与癌症相关的领域。作为一家始终致力于科学研究的公司，CST深知抗体的高特异性和批次间性能的一致性至关重要。因此我们坚持自己生产所有的抗体，并在多种应用中进行严格的验证。同时，正是生产这些抗体的CST科学家为客户提供技术支持，帮助他们设计实验，解决问题以获得最可靠的结果。我们之所以这样做，是因为如果我们做实验，我们也想要得到同样的服务。事实上，CST的确是这样的。
Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068

市场价

￥0.00

价格

￥0.00

品牌

CST

型号

13788

规格

1 Kit

客服