Cell Signaling(CST) 13047 PathScan® Th1/Th2/Th17 Cytokine Antibody Array Kit (Chemiluminescent Readout)

产品编号 13047 | Cell Signaling(CST)

Kit Includes	Quantity	Applications	Reactivity	Homology†	MW (kDa)	Isotype
Array Slides - Th1/Th2/Th17 Cytokine Array Kit	2 ea
10X Detection Antibody Cocktail - Th1/Th2/Th17 Cytokine Array Kit	300 µl
16-Well Gasket	2 ea
20X LumiGLO^®Reagent and 20X Peroxide #7003	5 ml each
HRP-Linked Streptavidin (10X)	300 µl
20X Array Wash Buffer	15 ml
Sealing Tape	2 sheets
Array Blocking Buffer	5 ml
Chemiluminescent Development Folder	2
Array Diluent Buffer	15 ml

PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Chemiluminescent Readout) detects the target proteins as specified on the Array Target Map (Figure 1). No significant cross reactivity has been observed between targets. This kit is optimized for cell culture supernatants. Recommended starting cell culture supernatant range is 20-75 μl. All antibodies have been validated for human-derived cell culture supernatants.

The PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Chemiluminescent Readout) uses glass slides as the planar surface and is based upon the sandwich immunoassay principle. This array kit allows for the simultaneous detection of 12 unique extracellular signaling molecules. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two slides allowing the user to test up to 32 different samples and generate 384 data points in a single experiment. Cell supernatant is incubated on the slide followed by a biotinylated detection antibody cocktail. Streptavidin-conjugated HRP and LumiGLO^® Reagent are then used to visualize the bound detection antibody by chemiluminescence. An image of the slide can be captured with either a digital imaging system or standard chemiluminescent film. The image can be analyzed visually or the spot intensities quantified using array analysis software.

Effective immune responses require finely tuned coordination between pro and anti-inflammatory signals. Pro-inflammatory molecules play important roles in activating key immune players to fight infection. IL-8 induces granulocyte migration and activates neutrophil phagocytic activity. GM-CSF mobilizes monocytes into infected tissue and activates macrophage and neutrophils. TNF-α is a multifunctional pro-inflammatory cytokine involved with a number of processes including cell proliferation, differentiation, and apoptosis.
Uncontrolled inflammation may damage surrounding host tissue. IL-10 is a prototypical anti-inflammatory cytokine that serves to terminate the acute inflammatory response by inhibiting Th1 cell function and pro-inflammatory cytokine production.

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CST公司是一家由研究型科学家创建于1999年的家族式私营企业，在全球范围内拥有超过400多名员工。CST活跃于应用系统生物学研究，尤其是与癌症相关的领域。作为一家始终致力于科学研究的公司，CST深知抗体的高特异性和批次间性能的一致性至关重要。因此我们坚持自己生产所有的抗体，并在多种应用中进行严格的验证。同时，正是生产这些抗体的CST科学家为客户提供技术支持，帮助他们设计实验，解决问题以获得最可靠的结果。我们之所以这样做，是因为如果我们做实验，我们也想要得到同样的服务。事实上，CST的确是这样的。
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价格

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品牌

CST

型号

13047

规格

1 Kit

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