Cell Signaling(CST) 12622 PathScan® EGFR Signaling Antibody Array Kit (Chemiluminescent Readout)

产品编号 12622 | Cell Signaling(CST)

产品描述

Kit Includes	Quantity	Applications	Reactivity	Homology†	MW (kDa)	Isotype
Array Slides - EGFR Signaling Array Kit	2 ea
Detection Ab Cocktail A(10X) - EGFR Signaling Array Kit	150 µl
Detection Ab Cocktail B(10X) - EGFR Signaling Array Kit	150 µl
16-Well Gasket	2 ea
20X LumiGLO^®Reagent and 20X Peroxide #7003	5 ml each
HRP-Linked Streptavidin (10X)	300 µl
Cell Lysis Buffer (10X) #9803	15 ml
20X Array Wash Buffer	15 ml
Sealing Tape	2 sheets
Array Blocking Buffer	5 ml
Chemiluminescent Development Folder	2
Array Diluent Buffer	15 ml

Specificity / Sensitivity

PathScan^® EGFR Signaling Antibody Array Kit (Chemiluminescent Readout) detects the target proteins as specified on the Array Target Map. No substantial cross-reactivity has been observed between targets. This kit is optimized for cell lysates diluted to a total protein concentration between 0.2 and 1 mg/ml (see kit protocol). All capture antibodies have been validated for human and mouse-derived samples.

Description

The PathScan^® EGFR Signaling Antibody Array Kit (Chemiluminescent Readout) uses glass slides as the planar surface and is based upon the sandwich immunoassay principle. The array kit allows for the simultaneous detection of phosphorylated EGFR, HER2, c-Met on distinct sites as well as a number of key signaling nodes found downstream of these receptor tyrosine kinases (RTKs). Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two slides allowing for the interrogation of 16 different samples. To improve assay performance the content of this array is split between two sub-arrays. The pads on left-hand side of each slide belong to sub-array A while the pads on the right-hand side of each slide belong to sub-array B. Cell lysates are incubated on the slide followed by a biotinylated detection antibody cocktail A or cocktail B (each cocktail for the corresponding sub-array). Streptavidin-conjugated HRP and LumiGLO^® Reagent are then used to visualize the bound detection antibody by chemiluminescence. An image of the slide can be captured with either a digital imaging system or standard chemiluminescent film. The image can be analyzed visually or the spot intensities quantified using array analysis software.

Background

The Epidermal Growth Factor Receptor (EGFR) is a receptor tyrosine kinase (RTK) that constitutes an important disease driver, as well as a validated drug target. The potency of EGFR in driving tumorigenesis can be attributed to its pleiotropic intracellular signaling. Activated EGFR initiates a wide range of signaling modules and switches such as the Ras-Erk/MAP kinase, Akt, Src, Stat, and PKC. Two of the most common EGFR mutations occurring in lung cancer are the E746-A750 deletion and L858R point mutation. This array utilizes unique antibodies made by Cell Signaling Technology that are sensitive to each of these EGFR mutants, allowing specific target detection in cell extracts.
EGFR can interact and heterodimerize with other RTKs. HER2 (also known as ErbB2) is an oncogenic RTK belonging to the EGFR/HER family of RTKs and is an important heterodimerization partner of all HER family members. Another prominent heterodimerization partner of EGFR is c-Met. c-Met is an RTK serving as a receptor for the hepatocyte growth factor (HGF). c-Met can induce cell scattering, migration, and invasion. It has been shown that c-Met is responsible for some cases of tumor resistance to EGFR-targeted therapies and is a contributing factor to tumor metastasis.
PLCγ is a phosphoinositide-specific phospholipase. EGFR can activate PLCγ that, in turn, hydrolyzes phosphoinositide phospholipids residing within the inner leaflet of the plasma membrane. This hydrolysis generates two important second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). IP3 causes calcium mobilization from intracellular storage pools, while DAG (together with calcium) activates PKC. MEK1 is a dual-specificity protein kinase and serves as the MAP kinase kinase for Erk1 and Erk2. Upon EGFR activation, MEK1 is phosphorylated by Raf and, in turn, phosphorylates the Erk kinases at Thr202 and Tyr204, leading to their activation. Activated Erk MAP kinase is a major signaling node with a multitude of substrates and primarily transmits growth and proliferation signals. Akt is another important protein kinase downstream of EGFR. Akt is activated by many RTKs and has a large number of intracellular substrates. Akt generates anabolic growth and survival signals. Stat3 is activated in response to EGFR stimulation, as well as in response to activation of a variety of cytokine receptors. Stat3 is a well-established oncogene that is also a transcription factor.
The oncogenic signals generated by activated EGFR are a focus of intense drug discovery efforts. It has become clear that in many cases a single agent inhibiting only one target is unable to cause tumor cell death in vivo. To monitor the blockade of EGFR signals alongside markers of cell death, cleaved PARP is included in this array. PARP is an enzyme involved in DNA repair. As a part of the apoptotic process, PARP is irreversibly inactivated by endoproteolytic cleavage executed by activated cell death proteases, such as caspase-3 and caspasae-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

12622 :

ELISA*

Companion Products

12785 PathScan^® EGFR Signaling Antibody Array Kit (Fluorescent Readout)

12856 PathScan^® Stress and Apoptosis Signaling Antibody Array Kit (Chemiluminescent Readout)

12923 PathScan^® Stress and Apoptosis Signaling Antibody Array Kit (Fluorescent Readout)

13047 PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Chemiluminescent Readout)

13124 PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Fluorescent Readout)

7323 PathScan^® Intracellular Signaling Array Kit (Chemiluminescent Readout)

7744 PathScan^® Intracellular Signaling Array Kit (Fluorescent Readout)

7949 PathScan^® RTK Signaling Antibody Array Kit (Fluorescent Readout)

7982 PathScan^® RTK Signaling Antibody Array Kit (Chemiluminescent Readout)

9474 PathScan^® Akt Signaling Antibody Array Kit (Chemiluminescent Readout)

9700 PathScan^® Akt Signaling Antibody Array Kit (Fluorescent Readout)

常见问题及解答

公司介绍

CST公司是一家由研究型科学家创建于1999年的家族式私营企业，在全球范围内拥有超过400多名员工。CST活跃于应用系统生物学研究，尤其是与癌症相关的领域。作为一家始终致力于科学研究的公司，CST深知抗体的高特异性和批次间性能的一致性至关重要。因此我们坚持自己生产所有的抗体，并在多种应用中进行严格的验证。同时，正是生产这些抗体的CST科学家为客户提供技术支持，帮助他们设计实验，解决问题以获得最可靠的结果。我们之所以这样做，是因为如果我们做实验，我们也想要得到同样的服务。事实上，CST的确是这样的。
Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068

市场价

￥0.00

价格

￥0.00

品牌

CST

型号

12622

规格

1 Kit

客服