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4242.00
Purified anti-HA.11 Epitope Tag
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Biolegend 901501 Purified anti-HA.11 Epitope Tag Antibody
产品编号 901501| biolegend
产品描述
Product Details | |
Clone: | 16B12 (See other available formats) |
Isotype: | Mouse IgG1, κ |
Isotype Control: | Purified Mouse IgG1, κ Isotype Ctrl |
Reactivity: | YPYDVPDYA Tag |
Immunogen: | Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL. |
Formulation: | Phosphate-buffered solution + 50% Glycerol + 0.03% Thimerosal. |
Preparation: | The antibody was purified by affinity chromatography. |
Concentration: | 1 mg/ml |
Storage & Handling: | Store between 2°C and 8°C. |
Application: | WB, IF, IP |
Recommended Usage: | Each lot of this antibody is quality control tested by Western blotting. |
The optimal working dilution should be determined for each specific assay condition. | |
• WB: 1:1,000* | |
• IF: 1:1,000 | |
• IP: 1:150 | |
Application | This antibody is effective in immunoblotting (WB), immunofluorescence (IF), and immunoprecipitation (IP) of tagged proteins. |
Notes: | |
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody. | |
This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. | |
Application | 1. Pecot MY, Malhotra V. 2004. Cell. 116:99. |
References: | 2. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB) |
3. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB) | |
4. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB) | |
5. Kolodziej P, Young R. 1991. Meth Enzymol. 194:508. (General) | |
6. Field J, et al. 1988. Mol Cell Biol. 8:2159. (General) | |
7. Majeed SR, et al. 2014. Nat Commun. 23:5891. (IF) PubMed | |
8. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed | |
9. Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed | |
10. Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed | |
11. Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed | |
12. Block E, et al. 2015. J. Neurosci.. 35: 12845-12858. PubMed | |
Publication Library | |
RRID: | AB_2565006 (BioLegend Cat. No. 901501) |
AB_2565007 (BioLegend Cat. No. 901502) | |
AB_2565005 (BioLegend Cat. No. 901503) | |
Antigen Details | |
Description: | The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA. |
Other Names: | HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag<mms-101p-200,mms-101p-500,mms-101p-1000 style="font-family: Arial, Helvetica, sans-serif; font-size: 14px; color: rgb(51, 51, 51);"> |
GeneID: | NA |
UniProt: | View information about HA.11 on UniProt.org |
Keywords: | Purified anti-HA.11 Epitope Tag, 16B12, Purified, HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag<mms-101p-200,mms-101p-500,mms-101p-1000 style="font-family: Arial, Helvetica, sans-serif; font-size: 14px; color: rgb(51, 51, 51);">, YPYDVPDYA Tag, Western Blotting, Immunofluorescence microscopy, Immunoprecipitation, Immunology, Antibodies |
公司简介
Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！
Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！
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市场价
￥4242.00
价格
￥4242.00
品牌
Biolegend(Covance)
型号
901501
规格
200 ul
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