Purified anti-Pax-6

￥ 4914.00

Purified anti-Pax-6

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Biolegend 901301 Purified anti-Pax-6 Antibody

产品编号 901301| biolegend

产品描述

Product Details
Clone:	Poly19013 (See other available formats)
Isotype:	Rabbit Polyclonal IgG
Reactivity:	Human, Mouse, Mammalian
Immunogen:	This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein.
Formulation:	Phosphate-buffered solution + 0.03% Thimerosal.
Preparation:	The antibody was purified by affinity chromatography.
Concentration:	2 mg/ml
Storage & Handling:	The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application:	WB, IHC, IF
Recommended Usage:	Each lot of this antibody is quality control tested by Western blotting and immunohistochemistry.
	The optimal working dilution should be determined for each specific assay condition.
	• Western blot: 1:200-500*
	• IHC: 1:50-1:100
	• IF: 1:300
	Tissue Sections: paraffin-embedded tissues, frozen sections
	Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (928501, 928601).
	Incubation: 60 minutes at room temperature
Application	This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF) and staining of tissue sections.
Notes:
	*Predicted MW = 47-50 kD
	Positive Control: human brain, epididymis
	The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a Protein A column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes.
Application	1. Tang K, et al. 2012. Development. 139:1630. (IF) PubMed
References:	2. Bandah D, et al. 2007. Invest. Ophthalmol. Vis. Sci. 48:2503. (WB) PubMed
	3. Marquardt T, et al. 2001. Cell. 105:43.
	4. Yamamoto Y, Jeffery WR. 2000. Science. 289:631
	5. Ashery-Padan R, et al. 2000. Genes Dev. 14:2701.
	6. Osumi N, et al. 1997. Development. 124:2961.
	7. Koroma BM, et al. 1997. Invest. Ophthalmol Vis Sci. 38:108.
	8. Davis JA, Reed RR. 1996. J Neurosci. 16:5082. (IHC)
	9. Small K, et al. 2015. Ophthalmology. 13: 681-685. PubMed
	10. Ahmad Z, et al. 2015. PLOS ONE. 10: 0144597. PubMed
	11. Verheyen A, et al. 2015. PLOS ONE. 10: 0146127. PubMed
	12. Bando Y, et al. 2016. Cereb Cortex. 26: 106 - 117. PubMed
	Publication Library
RRID:	AB_2565003 (BioLegend Cat. No. 901301)
Antigen Details
Description:	Pax6 is a transcription factor present during embryonic development. The encoded protein contains two different binding sites that are known to bind DNA and function as a regulator of gene transcription. It is a key regulatory gene of eye and brain development. Within the brain, the protein is involved in development of specialized cells that process smell. Pax-6 acts as a critical gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. Pax6 serves as a regulator in the coordination and pattern formation required for differentiation and proliferation to successfully take place, ensuring that the processes of neurogenesis and oculogenesis are carried out successfully. As a transcription factor, Pax6 acts at the molecular level in the signaling and formation of the central nervous system. The characteristic paired DNA binding domain of Pax6 utilizes two DNA-binding domains, the paired domain (PD), and the paired-type homeodomain (HD). These domains function separately. An example of this lies in HD’s regulatory involvement in the formation of the lens and retina throughout oculogenesis contrasted by the molecular mechanisms of control exhibited on the patterns of neurogenesis in brain development by PD. The HD and PD domains act in close coordination, giving Pax6 its multifunctional nature in directing molecular signaling in formation of the CNS.
	The vertebrate PAX6 locus encodes at least three different protein isoforms, these being the canonical PAX6, PAX6(5a), and PAX6(ΔPD). The canonical PAX6 protein contains an N-terminal paired domain, connected by a linker region to a paired-type homeodomain, and a proline/serine/threonine (P/S/T)-rich C-terminal domain. The paired domain and paired-type homeodomain each have DNA binding activities, while the P/S/T-rich domain possesses a transactivation function. PAX6(5a) is a product of the alternatively spliced exon 5a resulting in a 14 residue insertion in the paired domain which alters the specificity of this DNA binding activity. The nucleotide sequence corresponding to the linker region encodes a set of three alternative translation start codons from which the third PAX6 isoform originates. Collectively known as the PAX6(ΔPD) or pairedless isoforms, these three gene products all lack a paired domain. The pairedless proteins possess molecular weights of 43, 33, or 32kDa, depending on the particular start codon used. PAX6 transactivation function is attributed to the variable length C-terminal P/S/T-rich domain which stretches to 153 residues in human and mouse proteins.
Other Names:	Paired box protein Pax-6, oculorhombin, aniridia type II protein, Sey, Protein eyeless, PRB-278P-100
GeneID:	5080
UniProt:	View information about Pax-6 on UniProt.org
Keywords:	Purified anti-Pax-6, Poly19013, Purified, Paired box protein Pax-6, oculorhombin, aniridia type II protein, Sey, Protein eyeless, PRB-278P-100, Human, Mouse, Mammalian, Western Blotting, Immunohistochemistry, Immunofluorescence microscopy, Immunology, Antibodies

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￥4914.00

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￥4914.00

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Biolegend(Covance)

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