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4900.00
LEAF™ Purified anti-human CD3
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Biolegend 317315 LEAF™ Purified anti-human CD3 Antibody
产品编号 317315| biolegend
产品描述
Product Details | |
Clone: | OKT3 (See other available formats) |
Isotype: | Mouse IgG2a, κ |
Isotype Control: | LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl |
Reactivity: | Human |
Formulation: | 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test. |
Preparation: | The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography. |
Concentration: | 1.0 mg/ml |
Storage & Handling: | The CD3 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions. |
Application: | FC - Quality tested |
IHC, Activ - Reported in the literature | |
Recommended Usage: | Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Application | The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. |
Notes: | |
Clone OKT3 can block the binding of clones SK7 and UCHT1.4The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). | |
Application | 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. |
References: | 2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. |
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. | |
4. Li B, et al. 2005. Immunology 116:487. | |
5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed | |
6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed | |
7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed | |
8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed | |
9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed | |
10. Comrie WA, et al. 2015. J Cell Biol. 208:475. PubMed | |
11. Ulmansky R, et al. 2015. J Immunol. 194:5103. PubMed | |
12. Yu A, et al. 2015. Diabetes. 64:2172. PubMed | |
Publication Library | |
RRID: | AB_571924 (BioLegend Cat. No. 317303) |
AB_571925 (BioLegend Cat. No. 317304) | |
AB_1877070 (BioLegend Cat. No. 317315) | |
Antigen Details | |
Description: | CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. |
Other Names: | T3, CD3ε<cd3epsilon, cd3="" epsilon,="" cd3ε="" style="font-family: Arial, Helvetica, sans-serif; font-size: 14px; color: rgb(51, 51, 51);"> |
Structure: | Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD |
Distribution: | Mature T and NK T cells, thymocyte differentiation |
Function: | Antigen recognition, signal transduction, T cell activation |
Ligand Receptor: | Peptide antigen bound to MHC |
Antigen | 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. |
References: | 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. |
3. Lanier L, et al. 1986. J. Immunol. 137:2501. | |
GeneID: | 916 |
UniProt: | View information about CD3 on UniProt.org |
Keywords: | LEAF™ Purified anti-human CD3, OKT3, LEAF™, T3, CD3ε<cd3epsilon, cd3="" epsilon,="" cd3ε="" style="font-family: Arial, Helvetica, sans-serif; font-size: 14px; color: rgb(51, 51, 51);">, Human, Flow Cytometry, Immunohistochemistry, Activation, Biofunctional Assay, Immunology, Antibodies |
公司简介
Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！
Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！
流式细胞术简介：流式细胞术就是利用流式细胞仪对处在快速直线流动状态中的单列细胞或生物颗粒进行逐个、多参数、快速的定性、定量分析或分选的技术，具有检测速度快、测量指标多、采集数据量大、分析全面、方法灵活等特点。流式细胞术已被众多的研究人员作为优先选择的研究技术。目前，流式细胞术已广泛应用于免疫学、细胞生物学、遗传学、生物化学、肿瘤学、血液学等基础学研究和临床医学领域。
目前，Biolegend公司是世界上主要的流式抗体生产商，Biolegend公司作为后起之秀，以其过硬的质量、更低的价格、丰富的规格、优质的服务及快速的到货期为越来越多的科研工作者所青睐！
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市场价
￥4900.00
价格
￥4900.00
品牌
Biolegend
型号
317315
规格
1 mg
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