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Takara Clontech 7246 Human Mitochondrial DNA (mtDNA) Monitoring Primer Set
产品编号 7246| Takara Clontech
Human mitochondrial to nuclear DNA ratio kit
The Human Mitochondrial DNA (mtDNA) Monitoring Primer Set is designed to quantify the relative number of copies of human mitochondrial DNA (mtDNA) using nuclear DNA (nDNA) content as a standard by real-time PCR. This kit contains primer pairs for the amplification of four regions: two primer pairs for detecting mtDNA and two primer pairs for detecting nDNA. These primers will not amplify pseudogenes. As the primers are human-specific and will not amplify DNA from mice, human mtDNA content can be monitored in iPS cells or other cells cultured with feeder cells of mouse origin. This allows the Human Mitochondrial DNA (mtDNA) Monitoring Primer Set to be used in drug discovery applications where induced pluripotent stem cells (iPSCs) are used as a source of differentiated liver cells, since hepatocytes have a higher ratio of mtDNA to nuclear DNA than pluripotent cells.
Using the four primer pairs in this set for real-time PCR, relative quantificationof mtDNA is determined from the difference in Ct values for mtDNA and nDNA. This product provides a simple method for monitoring changes in the mtDNA copy number as mtDNA has been reported to increase during differentiation of pluripotent cells, such as iPS cells, into hepatic cells. The primer pairs in this set are compatible with Terra qPCR Direct SYBR Premix or SYBR Premix Ex Taq II (Tli RNaseH Plus).
产品描述
Features
ØIncludes primer sets for detection of two mtDNA targets (ND1, ND5) and two nDNA targets ( SLCO2B1, SERPINA1)
ØSpecific to human mtDNA; primer sets will not amplify psuedogenes
ØDesigned for use with Terra qPCR Direct SYBR Premix or SYBR Premix Ex Taq II (Tli RNaseH Plus)
Applications
ØMonitoring differentiation of hepatocytes induced from iPSC lines
ØQuantifying ratio of human mtDNA to human nuclear DNA
Components
ND1 Primer Mix (10 µM each) | 50 µL |
ND5 Primer Mix (10 µM each) | 50 µL |
SLCO2B1 Primer Mix (10 µM each) | 50 µL |
SERPINA1 Primer Mix (10 µM each) | 50 µL |
Storage
-20°C. Use within 1 year.
If using product repeatedly within the span of 1 month, store at 4°C. However, take care to avoid contamination as this product does not contain preservatives.
Data Analysis
To aid data analysis, please download and use the mtDNA_Copy_Number_Calculation tool (Excel spreadsheet) available on this web page (click on the Resources tab, then expand the Technical Notes drop-down menu).
Reference
Yue, Y.,et al. (2012) Hepatocyte-like cells differentiated from human induced pluripotent stem cells: Relevance to cellular therapies. Stem Cell Research9:196.
公司简介
美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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