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Takara Clontech T304 iMatrix-511
产品编号 T304| Takara Clontech
Stem cell culture reagent: iMatrix-511 (recombinant laminin-511)
Propagation of embryonic stem (ES) cells or induced pluripotent stem (iPS) cells requires a culture substrate that retains pluripotency, stable karyotype, and expression of pluripotent markers. iMatrix-511 is a recombinant laminin-511 E8 fragment product that can be used as an ES or iPS cell culture substrate. These recombinant laminin-511 E8 fragments permit maintenance of human ES and iPS cells in xeno-free culture conditions and provide greater adhesive properties than either full-length laminin, vitronectin, or undefined basement membrane reagents such as Matrigel. The iMatrix-511 cell culture reagent allows stem cell culture in absence of mouse feeder cells.
Recombinant laminin-511 E8 fragments are truncated, functional forms of laminin composed of the C-terminal regions of the alpha, beta, and gamma chains. Laminin-511 E8 (150 kDa) retains the full capability for binding to alpha 6 beta 1 integrin. Based on research performed at Kyoto University and Osaka University, recombinant laminin-511 E8 fragments are available as iMatrix-511. This product is available through an agreement between Takara Bio Inc. and Nippi, Inc.
产品描述
FeaturesChemically-defined stem cell culture substrate for animal-free, xeno-free, feeder-free ES or iPS cell culture Provides greater adhesion of human ES and iPS cells than Matrigel or full-length laminin Laminin-511 E8 fragments sustain long-term self-renewal of human ES and iPS cells in defined xeno-free media with dissociated cell passaging Promotes high expression of pluripotency markers, normal karyotype, and retained ability to differentiate into all three germ layers ApplicationsStem cell culture under chemically defined, animal-free, xeno-free conditions Expansion culture of ES and iPS cells ComponentsiMatrix is available in both lyophilized and liquid formats: Cat. #T300: 350 ug recombinant laminin-511 E8 fragments (lyophilized) Cat. #T303:350 ug recombinant laminin-511 E8 fragments (liquid, 0.5 mg/ml) Cat. #T304:1,050 ug recombinant laminin-511 E8 fragments (liquid, 0.5 mg/ml) References1. Miyazaki, T., et al. (2012) Laminin E7 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3:1236. 2. Taniguchi, Y., et al. (2009) The C-terminal region of laminin beta chains modulates the integrin binding affinities of laminins. J. Biol. Chem. 284(12):7820. 3. Ido, H., et al. (2007) The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma chains in integrin binding by laminins. J. Biol. Chem. 282(15):11144. Additional InformationPlease refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications. |
公司简介
美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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