Takara Clontech Y40001 RHB-A®

产品编号  Y40001 | Takara Clontech

Neural Stem Cell Culture Media

• RHB-A and RHB-Basal is a fully defined, serum-free cell culture medium for the derivation, maintenance, expansion, and differentiation of human and mouse neural stem (NS) cells. RHB-A is specifically formulated for culturing pure populations of adherent human and mouse NS cells. This optimized medium ensures the long-term stability of NS cell differentiation capacity (1) and can be used to support differentiation of human and mouse NS cells into functional neurons (1–3) and of mouse ES cells into neural precursors (4–6).
• RHB‐Basal does not contain any growth factors or neuronal supplements.  Therefore, this media can be tailored to the specific requirements of your cell type by the addition of supplements.

产品描述

Features

RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells

RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be customized by the addition of supplements

Applications

Derivation of mouse and human NS cells from ES cells and fetal and adult tissues

Maintenance and propagation of adherent mouse and human NS cells

Differentiation of mouse and human NS cells into functional neurons

Differentiation of mouse ES cells to neuronal precursors

Refer to the Data Sheet for additional examples of use

Components

500 ml medium

References

Sun, Y. et al. (2008) Long‐term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Molecular and Cellular Neuroscience 38:245–258

Conti, L. et al. (2005) Niche‐Independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biology 3(9):e283.

Hansen, D.V. et al. (2010) Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature 464(7288):554–561.

Ying, Q.L. et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nature Biotechnology 21:183–186.

Diogo, M.M. et al. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Biotechnology and Applied Biochemistry49:105–112.

Abranches, E. et al. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis in the embryo. PLoS ONE 4(7):e6286.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.

公司简介

美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。

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