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Takara Clontech  PH017    PrimerArray® Hepatic Differentiation (Human)    

产品编号  PH017 | Takara Clontech

RT-PCR Primers to Analyze Hepatocyte Differentation

PrimerArray Hepatic Differentiation (Human) is a set of real-time RT-PCR primers for the analysis of the expression of genes associated with hepatocyte differentiation. The array contains primer pairs optimized for real-time RT-PCR for the analysis of 88 pluripotency markers, endoderm markers, and liver cell markers and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. Data analysis is aided by use of the PrimerArray Analysis Tool for Hepatic Differentiation, available for download under the Resources tab on this web page.

产品描述

Features

ØRT-PCR primers arrayed in 96-well plate allows  rapid assessment of induction and differentiation of pluripotent cells  into hepatocytes

ØMinimizes pipetting and reduces error

ØEnables high-throughput analysis of agents affecting hepatocyte differentiation

ØOptimized for superior performance with SYBR Ex TaqqPCR kits

ØPrimerArray Analysis Tool makes data analysis easy

ØCompatible with all standard real-time PCR instrument systems

Applications

ØMonitoring hepatocyte differentiation/induction

ØScreening for expression of hepatocyte cell marker genes in response to conditions or agents affecting differentiation

Procedural Overview for Hepatocyte Differentiation Gene Expression Analysis by Real Time RT-PCR

The overall workflow for the PrimerArray Series primer sets is straightforward:

1 RNA extraction(30 min): We recommend using NucleoSpin RNA (Cat. #740955.50) or RNAiso Plus (Cat.# 9108/9109, not available in all geographic locations). Removal of genomic DNA using Recombinant DNase I (RNase-free) (Cat.# 2270A) is recommended.

2 Reverse transcription(20 min): PrimeScript RT Master Mix (Perfect Real Time) (Cat. #RR036A) is recommended.

3 Dispense real-time PCR reaction solution(10 min): Prepare a master mix containing the cDNA sample and SYBR Premix Ex TaqII (Tli RNase H Plus) (Cat.# RR820A); dispense into 96-well plate.

4 Add PrimerArray primers(5 min): Use 4 µL PrimerArray Series primers per reaction  

5 Real-time PCR(1 - 2 hours): PrimerArray Series primers are compatible with all commonly used real-time PCR systems  

6 Data analysis(30 minutes): Use the PrimerArray Analysis Tool

PrimerArray Analysis Tool for Hepatic Differentiation (Human)

The PrimerArray analysis tool facilitates data analysis and can be downloaded from this web page. To download both the Analysis Tool and an instruction guide about how to use it, click on the Resources tab, then expand the drop-down menu under Technical Notes.

Information about RT-PCR Primers for PrimerArray Hepatic Differentiation (Human)

To download primer information for this set of PrimerArray RT-PCR primers, click on the Resources tab, then expand the drop-down menu under Technical Notes.

公司简介

美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

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