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Takara Clontech RR290 Mouse Feeder Cell Quantification Kit
产品编号 RR290| Takara Clontech
Detection of mouse feeder cell contamination in stem cell cultures
The Mouse Feeder Cell Quantification Kit is designed to assess stem cell culture quality by measuring mouse feeder cell contamination in stem cell cultures. Mouse feeder cells are used to support the growth and cultivation of stem cells such as hESC (human embryonic stem cells) and hiPSC (human induced pluripotent stem cells)—but they must be removed before use in certain applications, such as regenerative medicine. This kit provides highly sensitive detection and quantification of genomic DNA derived from residual mouse feeder cells by using real-time PCR to analyze genomic DNA extracted from stem cell cultures.
The Mouse Feeder Cell Quantification Kit includes real-time PCR primers that detect a mouse mitochondrial (mt) DNA sequence, providing highly sensitive detection of mouse feeder cells. The kit also includes nuclear DNA PCR primers for normalization in order to quantify the mouse mtDNA copy number, which can vary between different mouse feeder cell lots, depending on the cell type, cell line, and differentiation state. Calculating the mouse mtDNAcopy number makes it possible to compare the levels of mouse cell contamination in stem cell cultures grown with different lots of mouse feeder cells.
产品描述
FeaturesUtilizes real-time PCR to detect and quantify residual mouse feeder cells Provides mtDNA PCR primers to make mouse mtDNA sequence detection easy and sensitive Includes nuclear DNA PCR primers for normalization to quantify mouse mtDNA copy number ApplicationsAssess the level of mouse cell contamination in stem cell cultures grown using mouse feeder cells Compare the levels of mouse cell contamination in stem cell cultures grown with different lots of mouse feeder cells ComponentsStorage4°C (SYBR Premix Ex Taq II (Tli RNaseH Plus) Product is stable for up to 6 months when stored at 4°C. Protect from light and avoid contamination. This kit is shipped in a dry ice-packed container. For long-term storage, keep item at –80°C. Do not store at –20°C. Store thawed product at 4°C and use within 6 months. –20°C (all other components) |
公司简介
美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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