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Takara Clontech 631965 Pluripotency Check PCR Kit
产品编号 631965 | Takara Clontech
Stem Cell Pluripotency Status Check Kit with PCR Primers
The Pluripotency Check PCR Kit contains all the necessary reagents to confirm that your mouse stem or iPS cells are indeed pluripotent. It includes 16 prevalidated forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes. The kit includes everything for start-to-finish confirmation of your cells’ pluripotency status, using any combination of primer sets, or the entire panel.
The Pluripotency Check PCR Primer Set consists of 16 prevalidated primer mixes based on nine genes expressed in pluripotent stem cells, plus two control genes. The different amplicon sizes allow you to distinguish between endogenous and exogenous expression.
Pluripotency Check PCR Primers
Primer set | Amplicon size | Primer set | Amplicon size |
Oct3/4 (Total) | 485 bp | Klf4 | 739 bp |
Oct3/4 (Endogenous) | 224 bp | Klf4 (Endogenous) | 206 bp |
Nanog(Total) | 364 bp | Ecat1 | 164 bp |
Nanog (Endogenous) | 223 bp | ERas1 | 210 bp |
Sox2 (Total) | 193 bp | Esg1 | 376 bp |
Sox2 (Endogenous) | 297 bp | Rex1 | 287 bp |
c-Myc (Total) | 228 bp | Beta-Actin (Control) | 603 bp |
c-Myc (Endogenous) | 170 bp | GAPDH (Control) | 568 bp |
产品描述
Features
Validate the pluripotent status of your stem cell or iPS cell culture using a simple PCR kit
Ø16 prevalidated PCR primer mixes
ØComplete kit with RNA extraction, reverse transcription, and PCR reagents
ØConfirm your iPS cells by differentiating between endogenous and exogenous gene expression
Applications
ØValidating mouse embryonic stem cell status
ØConfirming iPS status
Additional Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
公司简介
美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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重庆市寰宇安泰生物科技有限公司
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