Takara Clontech Y40010 iSTEM™

产品编号  Y40010 | Takara Clontech

Mouse Embryonic Stem Cell Culture Medium: iSTEM Medium

• iSTEM is a completely defined cell culture medium for deriving, maintaining, and propagating mouse embryonic stem (ES) cells. This media formulation captures mouse ES cells in their basal state by blocking the inductive pathways of differentiation with selective small molecule inhibitors. The medium contains three small molecule inhibitors that block differentiation-inducing signals and promote cell survival, enabling maintenance of pluripotency without the need for additional stimulatory cytokines. iSTEM medium can also be used to derive mouse ES lines from strains that have proven refractory to ES cell derivation under standard conditions (1).
• Mouse ES cells cultured in iSTEM medium continually expand at a doubling rate that is comparable to that of cells cultured in the presence of Leukemia Inhibitory Factor (LIF) and serum/Bone Morphogenetic Protein (BMP) (1). In addition, culture in iSTEM medium enhances clonogenicity; a higher frequency of pluripotent colonies are established after single cell deposition.

产品描述

Features

Completely defined formulation: serum-free, feeder-free, and cytokine-free

Uses selective small-molecule inhibitors to block differentiation signals and maintain ES cells in a pluripotent state

Using iSTEM medium, mouse ES cells can be efficiently maintained and propagated at ≥99% purity

Applications

Maintaining mouse ES cells in a pluripotent state

Deriving mouse ES cell lines from non-permissive mouse strains

Components

100 ml medium

100 µl supplement

References

Ying, Q. L. et al. (2008) The ground state of embryonic stem cell self-renewal. Nature453:519–523.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.

公司简介

美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。

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