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Takara Clontech 632010 iPS Efficiency Check qPCR Primer Set
产品编号 632010 | Takara Clontech
iPS Efficiency Check qPCR Kit and Primer Set
The iPS Efficiency Check qPCR Kit lets you easily quantitate expression levels of key senescence markers to help you determine somatic cell reprogrammability, senescence marker expression levels (before and during reprogramming), and whether siRNA or knockdown construct(s) are required to enhance the efficiency of induced pluripotent stem (iPS) cell generation.
The kit provides all of the reagents needed to assess your target cell’s reprogrammability. It features prevalidated, forward and reverse primer mixes specific for four key senescence markers and one housekeeping (control) gene (Table I.) It also includes all of the reagents necessary for RNA extraction, first-strand cDNA synthesis, and quantitative real-time PCR. We also offer the iPS Efficiency Check qPCR Primer Set—which simply contains the prevalidated, forward and reverse primer mixes listed in Table I—for easy integration into your lab's existing workflow.
Table I. iPS Efficiency Check qPCR Primers | |
Primer Set | Amplicon Size |
Arf (senescence marker) | 67 bp |
Ink4a (senescence marker) | 59 bp |
p21 (senescence marker) | 140 bp |
p53 (senescence marker) | 109 bp |
Beta-Actin (control) | 151 bp |
产品描述
Features
ØPrevalidated qPCR primer mixes specific for key senescence markers
ØComplete kit to verify iPS generation: includes RNA extraction, reverse transcription, and qPCR reagents
Applications
ØDetermine the reprogrammability of somatic cells before and during cell reprogramming
ØMeasure senescence markers Arf, Ink4a, p21 and p53
Additional Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
公司简介
美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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