R&D  HSC003  Human Methylcellulose Complete Media

产品编号 HSC003 | rndsystems

产品介绍

Kit Summary

For the differentiation and enumeration of human hematopoietic stem cells, optimized with premium quality cytokines.

Key Benefits

Does not require addition of serum or cytokines

Optimized with premium quality recombinant proteins

Excellent optical clarity facilitates colony identification

High lot-to-lot consistency decreases variation

Why use R&D Systems Human Methylcellulose Complete Media for Colony Forming Cell Assays?

• Colony forming cell (CFC) assays, which are used to enumerate and quantify multi-potent and single lineage hematopoietic progenitors, can be time consuming and laborious.  

Components

100 mL of Human Methylcellulose Complete Media and 15 mL of Cell Resuspension Solution.  

Data Examples

• Human Hematopoietic Colony Formation Using the Methylcellulose-based Colony Forming Cell Assay. A. Colony forming unit-erythroid (CFU-E) are clonogenic progenitors that produce only one or two clusters with each cluster containing from 8 to approximately 100 hemoglobinized erythroblasts. It represents the more mature erythroid progenitors that have less proliferative capacity. B. Colony forming unit-granulocyte (CFU-G) are clonogenic progenitors of granulocytes that give rise to a homogeneous population of eosinophils, basophils, or neutrophils.C. Colony forming unit-granulocyte, macrophage (CFU-GM) are progenitors that give rise to colonies containing a heterogeneous population of macrophages and granulocytes. The morphology is similar to the CFU-M and CFU-G descriptions. D. Burst forming unit-erythroid (BFU-E) colonies can be described as small (3 to 8 clusters), intermediate (9 to 16 clusters), or large (more than 16 clusters) according to the number of clusters present. These are primitive erythroid progenitors that have high proliferative capacity. E. Colony forming unit-macrophage (CFU-M) are clonogenic progenitors of macrophages that give rise to a homogenous population of macrophages. F. Colony forming unit-granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM) are multi-lineage progenitors that give rise to erythroid, granulocyte, macrophage and megakaryocyte lineages, as the name indicates.

Guide to Choosing Media for the Colony Forming Cell (CFC) Assay

Human Methylcellulose Stock and Base Media




Catalog #	Product Description	Volume	ColoniesSelected for	ContainsSerum	CytokinesIncluded
HSC001	MethylcelluloseStock Solution	100 mL	N/A*	No	None
HSC002	Human MethylcelluloseBase Media	90 mL	N/A*	Yes	None
HSC002SF	Human MethylcelluloseSerum-Free Base Media	90 mL	N/A*	No	None
HSC011	StemXVivo™ MethylcelluloseConcentrate	50 mL	N/A*	No	None




Complete Human Methylcellulose Media




Catalog #	Product Description	Volume	ColoniesSelected for	ContainsSerum	CytokinesIncluded
HSC003	Human MethylcelluloseComplete Media	100 mL	BFU-ECFU-ECFU-GCFU-GEMMCFU-GMCFU-M	Yes	EpoGM-CSFIL-3SCF
HSC004	Human MethylcelluloseComplete Media without Epo	100 mL	CFU-GCFU-GMCFU-M	Yes	SCFGM-CSFIL-3
HSC005	Human MethylcelluloseEnriched Media	100 mL	BFU-ECFU-ECFU-GCFU-GEMMCFU-GMCFU-M	Yes	EpoG-CSFGM-CSFIL-3IL-6SCF
HSC005SF	Human MethylcelluloseSerum-Free Enriched Media	100 mL	BFU-ECFU-ECFU-GCFU-GEMMCFU-GMCFU-M	No	EpoG-CSFGM-CSFIL-3IL-6SCF
HSC010SF	Human MethylcelluloseSerum-Free Enriched Mediawithout Epo	100 mL	CFU-GCFU-GMCFU-M	No	G-CSFGM-CSFIL-3IL-6SCF




• *Base media and stock solutions do not contain cytokines and will not support colony growth unless conditioned media, cytokines, or other culture supplements are added.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Hematopoietic Stem Cells

• The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long maintenance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continuously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage depletion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

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