R&D SC028 Human/Mouse/Rat Neural Lineage Functional Identification Kit

产品编号 SC028 | rndsystems

HHuman/Mouse/Rat Neural Lineage Functional Identification Kit

产品介绍

Product Details

To verify the multipotency of neural progenitor cells (NPCs) by in vitro functional differentiation.

Key Benefits

Confirms that the starting population is multipotent

Reduces experimental variation

Assesses the differentiation of NPCs into astrocytes, neurons, and oligodendrocytes

Why Functionally Verify Neural Progenitor Cell Multipotency In Vitro?

Neural stem cell research studies most often require weeks of cell culture before an experimental hypothesis can be tested.

The ability to verify multipotency at an early stage in the experimental design provides valuable insight that may prevent weeks of wasted effort and reagents. Moreover, confirmation of multipotency helps to ensure consistency among studies and reduce unwanted experimental variability. Neural progenitor cell (NPC) multipotency is best evaluated by functionally assessing the ability of NPCs to differentiate into multiple neural lineages.

Functional verification of NPC multipotency in vitro:

Uses a specially formulated supplement to drive reproducible differentiation.

Verifies a healthy, multipotent starting NPC population to increase consistency between studies and reduce unwanted experimental variability.

Provides results in 7-10 days to save time and reagents.

Uses a monolayer culture system, which, compared to neurosphere systems, allows direct observation of NPCs and differentiated derivatives.

Kit Contents

This kit contains the following reagents to drive NPC differentiation and a marker to analyze NPCs and each of the three differentiated derivatives:

Neural Maintenance Supplement (10X)

Neural Differentiation Supplement

Bovine Fibronectin (100X)

NPC Marker: Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody

Astrocyte Marker: Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody

Neuron Marker: Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody

Oligodendrocyte Marker: Mouse Anti-Oligodendrocyte Marker O4 Monoclonal Antibody

The quantity of each component in this kit is sufficient to differentiate two 24-well plates, or an equivalent surface area, of pluripotent stem cells into hepatocyte-like cells

Data Examples

Verification of Neural Progenitor Cell Multipotency.Rat neural progenitor cells were maintained in culture and differentiated towards neural lineages using the specialized media supplement supplied in the Human/Mouse/Rat Neural Lineage Functional Identification Kit (Catalog # SC028). Neural progenitor cell multipotency was functionally verified using antibodies (also supplied in the kit) to detect phenotype-specific markers for undifferentiated neural precursors and the following differentiated derivatives: astrocytes, neurons, and oligodendrocytes. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Preparation and Storage

Stability & Storage

Background: Neural Stem Cells

Neural stem cells provide an excellent model for research focused on neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. In addition, primary mouse cortical stem cells isolated from E14.5 CD-1 mice are available. Every lot of R&D Systems Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. Our cortical stem cells are tested to ensure highest quality and lot to lot consistency. Both rat and mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.

To complement the use of primary neural stem cells, we offer a range of supportive products, including culture media which is specifically optimized for use with neural stem cells. We offer kits to promote the in vitro proliferation of neural precursors, and kits to differentiate neural stem cells into dopaminergic neurons or oligodendrocytes. In addition, kits are available which contain panels of antibodies designed to monitor the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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