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R&D HSC011 StemXVivo Methylcellulose Concentrate

产品编号 HSC011 | rndsystems

StemXVivo Methylcellulose Concentrate

产品介绍

Product Details

Kit Summary

For the growth and differentiation of human, mouse, and rat hematopoietic stem cells using Iscove’s Modified Dulbecco’s Media or any other basal media.

ØIs not restricted to use with Iscove's Modified Dulbecco's Media (IMDM)

ØSupports reproducible in vitrogrowth of hematopoietic stem and progenitor cells

ØCan be supplemented with user-defined cytokines and growth factors

ØExcellent optical clarity facilitates colony identification

ØHigh lot-to-lot consistency decreases variation

Why Make Methylcellulose Media from a Concentrated Stock Solution Rather than a Powder?

Although Methylcellulose is soluble at low temperatures, adding powdered methylcellulose to cold water results in clumping of the methylcellulose rather than its dissolution.

Using hot water to dissolve methylcellulose is also not an option since methylcellulose is insoluble in hot water. Therefore, methylcellulose stock solutions are made by adding methylcellulose to hot water (~80 °C) and then decreasing the temperature of the solution until the methylcellulose is fully dissolved. To avoid this time consuming and potentially inconsistent process, R&D Systems offers high quality StemXVivo™ Methylcellulose Concentrate with superior optical clarity to support optimal colony growth, enumeration, and identification.

R&D Systems Human Methylcellulose Serum-Free Base Media:

ØOptical clarity facilitates colony identification.

ØHigh lot-to-lot consistency decreases variation.

ØSupports reproducible in vitrogrowth of hematopoietic stem and progenitor cells.

ØCan be supplemented with user-defined cytokines and growth factors.

ØIncreased cloning efficiency and improved colony growth compared to agar.

Components

Ø50 mL of 2.8% Methylcellulose (1500 cps) in water.

Storage

The Methylcellulose Concentrate should be stored at =-20 °C upon receipt. Storage at 2 °C to 8 °C is not recommended.

Precaution

The acute and chronic effects of overexposure to this media are unknown. Safe laboratory procedures should be followed and protective clothing should be worn when handling this media.

Limitations of the Procedure

ØThe safety and efficacy of this product in diagnostic or other clinical uses has not been established.

ØThe reagent should not be used beyond the expiration date indicated on the label.

ØResults may vary due to variations between hematopoietic progenitors derived from different individuals.

Data Examples

Human Hematopoietic Colony Formation Using the Methylcellulose-based Colony Forming Cell Assay. A. Colony forming unit-erythroid (CFU-E) are clonogenic progenitors that produce only one or two clusters with each cluster containing from 8 to approximately 100 hemoglobinized erythroblasts. It represents the more mature erythroid progenitors that have less proliferative capacity. B. Colony forming unit-granulocyte (CFU-G) are clonogenic progenitors of granulocytes that give rise to a homogeneous population of eosinophils, basophils, or neutrophils. C. Colony forming unit-granulocyte, macrophage (CFU-GM) are progenitors that give rise to colonies containing a heterogeneous population of macrophages and granulocytes. The morphology is similar to the CFU-M and CFU-G descriptions. D. Burst forming unit-erythroid (BFU-E) colonies can be described as small (3 to 8 clusters), intermediate (9 to 16 clusters), or large (more than 16 clusters) according to the number of clusters present. These are primitive erythroid progenitors that have high proliferative capacity. E. Colony forming unit-macrophage (CFU-M) are clonogenic progenitors of macrophages that give rise to a homogenous population of macrophages. F. Colony forming unit-granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM) are multi-lineage progenitors that give rise to erythroid, granulocyte, macrophage and megakaryocyte lineages, as the name indicates.

Mouse Hematopoietic Colony Formation Using the Methylcellulose-based Colony Forming Cell Assay. A.Burst forming unit-erythroid (BFU-E) colonies are defined as clusters with a minimal of 30 cells that can be seen from day 7 onward. Each individual cluster consisted of tiny, irregular shaped cells that may appear fused together. Each cluster normally contains 5 - 8 cells, and the size of the cluster is similar to that of a single macrophage. The cluster may vary in sizes and color. A large BFU-E is usually bright red and is differentiable even without the use of a microscope. Smaller BFU-E may not appear red in color but is distinguishable based on the morphology. B.Colony forming unit-macrophage (CFU-M; left) are clonogenic progenitors of macrophages that give rise to a homogenous population of macrophages. Colony forming unit-granulocyte (CFU-G; right) are clonogenic progenitors of granulocytes that give rise to a homogeneous population of eosinophils, basophils, or neutrophils. C. Colony forming unit-granulocyte, macrophage (CFU-GM) are progenitors that give rise to colonies containing a heterogeneous population of macrophages and granulocytes. The morphology is similar to the CFU-M and CFU-G descriptions.D.Colony forming unit-granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM) are multi-lineage progenitors that give rise to the lineage of erythroid, granulocytes, macrophages, and megakaryocytes as the name indicates. It can be identified as reddish colored cells (erythroid) mixed with colorless cells (granulocytes, macrophages, and megakaryocytes) in a single colony. This progenitor is typically the largest colony on the culture dish; occasionally CFU-GM may attain a size comparable or larger than that of CFU-GEMM.

Guide to Choosing Media for the Colony Forming Cell (CFC) Assay

Human Methylcellulose Stock and Base Media

Complete Human Methylcellulose Media

Mouse Methylcellulose Stock and Base Media

Complete Mouse Methylcellulose Media

*Base media and stock solutions do not contain cytokines and will not support colony growth unless conditioned media, cytokines, or other culture supplements are added.

Preparation and Storage

ØStability & Storage

ØStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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