- 产品详情
- 产品参数
- 产品评论
R&D CCM010 StemXVivo Serum-Free Human T Cell Base Media
产品编号 CCM010| rndsystems
StemXVivo Serum-Free Human T Cell Base Media
产品介绍
Kit Summary
Pre-optimized for ex vivo culture of human T lymphocytes.
Key Benefits
Supports T lymphocyte expansion as well as or better than RPMI containing FBS
Defined media decreases experimental variation
High lot-to-lot consistency decreases variation
Why use pre-optimized media for ex vivo culture of human T lymphocytes?
Isolation of human T-lymphocytes from peripheral blood is a time consuming process that can yield cells of varying purity and quality.
Once in culture, the maintenance and expansion of T lymphocytes is highly dependent on the media, growth factors, and cytokines. R&D Systems offers Human StemXVivo Serum-Free T Cell Base Media, a defined media that outperforms RPMI containing Fetal Bovine Serum in its ability to support T cell expansion.
Human StemXVivo Serum-Free T Cell Base Media:
Supports T lymphocyte expansion better than RPMI containing 10% FBS.
Decreases experimental variability through the use of defined media components.
Can be used for multiple applications including mixed lymphocyte reactions (MLR), expansion of antigen-specific or non-specific T cells, and proliferation of peripheral blood lymphocytes in the presence of pharmacological agents such as PMA or ConA
Contents
250 mL StemXVivo Serum-Free T Cell Base Media.
The quantity of each component in this kit is sufficient to differentiate two 24-well plates, or an equivalent surface area, of pluripotent stem cells into hepatocyte-like cells
Stability and Storage
Upon receipt, the Human StemXVivo Serum-Free T Cell Base Media should be stored at =-20℃ in a manual defrost freezer. The media can be thawed at 2 ℃ to 8 ℃ or at room temperature. Thawed media can be aliquoted and stored at =-20 ℃ in a manual defrost freezer until the expiration date on the vial label or used within a month when stored in the dark at 2 ℃ to 8 ℃. Avoid repeated freeze-thaw cycles.
Precautions
The human origin-derived components used in this product have been derived from human plasma, which has been tested and found negative for antibodies to HIV-1/2, hepatitis B surface antigen (HBsAg), and hepatitis C virus (HCV). However, the medium should be handled as if potentially infectious. Safe laboratory procedures should be followed and protective clothing should be worn when handling this media. The acute and chronic effects of over-exposure to this media are unknown.
Limitations
FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
This reagent should not be used beyond the expiration date indicated on the label.
Results may vary due to variations among primary T lymphocyte populations derived from different donors.
Data Examples
Proliferative Response of Cultured CD3/CD28 Primed T cells in Human StemXVivo Serum-Free T Cell Base Media. 1 x 105 purified CD3+ T cells were cultured for five days in StemXVivo Serum-Free T Cell Base Media (Catalog # CCM010) or with RPMI supplemented with 10% Fetal Bovine Serum (RPMI/10% FBS). The purified CD3+ T cells were cultured on 96 well microplates coated with Mouse Anti-Human CD3 epsilon (Clone UCHT1; Catalog # MAB100) and Goat Anti-Human CD28 Antigen Affinity-purified Polyclonal Antibody. [3H]-thymidine was added for the final 18 hours. Cells were harvested and the incorporation of [3
Phenotypic Analysis of Cultured Monocyte-derived Dendritic Cells Before and After LPS-induced Maturation. Immature monocyte-derived dendritic cells (MoDCs) (open histograms; green line) were obtained after CD14+-enriched monocytes were cultured for seven days in StemXVivo Serum-Free Dendritic Cell Base Media (Catalog # CCM003) supplemented with 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) and 35 ng/mL Recombinant Human IL-4 (Catalog # 204-IL). Mature monocyte-derived dendritic cells (filled histograms) were cultured under the same conditions for seven days and then induced with LPS for an additional 48 hours. Day seven immature MoDCs and day nine LPS-treated MoDCs were stained with a FITC-conjugated Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # FAB140F), a FITC-conjugated Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # FAB1774F), a FITC-conjugated Mouse Anti-Human B7-2/CD86 Monoclonal Antibody (Catalog # FAB141F), an Anti-MHC Class II Antibody, or an appropriate isotype control antibody (empty histogram with solid line).
Mature Monocyte-derived Dendritic Cells Induce Proliferation of Allogenic T Cells. CD14+ monocytes were cultured for seven days in Human StemXVivo Serum-free Dendritic Cell Base Media (Catalog # CCM003) supplemented with Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human IL-4 (Catalog # 204-IL) and Gentamycin. The cells were subsequently treated with LPS for an additional 48 hours to induce dendritic cell maturation. Graded doses of mature monocyte-derived dendritic cells were incubated with 1 x 105 autologous or allogenic CD3+ T cells for five days. 3H-thymidine (3H-TdR) was added to the culture for the final 18 hours and T cell proliferation was measured using a scintillation counter. Results are presented as the mean cpm obtained from three experiments. |
Preparation and Storage
Stability & Storage
Store the unopened product at -20 to -70 ℃. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Hematopoietic Stem Cells
The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long mainte nance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continu ously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage deple tion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.
Related Research Areas
Hematopoietic Stem Cell Markers
公司简介
R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。
目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。
Uante,您的科研物资战略合作伙伴！
重庆市寰宇安泰生物科技有限公司
固定电话：023-67946738
全国热线：400-8623-068