综合电商模板

R&D  CCM003  StemXVivo Serum-Free Dendritic Cell Base Media

产品编号 CCM003| rndsystems

StemXVivo Serum-Free Dendritic Cell Base Media

产品介绍

Kit Summary

Pre-optimized base media for successful dendritic cell culture.

Key Benefits

Pre-optimized media for dendritic cell culture

High lot-to-lot consistency decreases variation

Can be supplemented with user-defined cytokines and growth factors

Why Use Pre-optimized Reagents for the Production of Dendritic Cells from Monocytes and Subsequent Dendritic Cell Culture?

The efficiency of differentiation of common myeloid progenitors into dendritic cells can vary from one experiment to another depending on the media components, the supplements used to drive differentiation, and the quality of the starting cell population.

Inefficient differentiation represents a costly and time-consuming obstacle that can be minimized through the use of high quality media and differentiation supplements. StemXVivo Serum-Free Dendritic Cell Base Media is a defined media optimized for dendritic cell culture. The addition of Recombinant Human GM-CSF (Catalog # 215-GM) and Recombinant Human IL-4 (Catalog # 204-IL) generates a defined medium that efficiently drives differentiation of CD14+ monocytes into monocyte-derived dendritic cells (MoDCs).

StemXVivo Serum-Free Dendritic Cell Base Media:

Optimized for dendritic cell culture.

High lot-to-lot consistency decreases variation.

Can be supplemented with user-defined growth factors and cytokines.

Optimized for differentiation of CD14+ monocytes into dendritic cells.

聽

Media Components

Reagents supplied in the StemXVivo Serum-Free Dendritic Cell Base Media (Catalog # CCM003):

250 mL StemXVivo Serum-Free Dendritic Cell Base Media

Stability and Storage

Upon receipt, the Human StemXVivo Serum-Free Dendritic Cell Base Media should be stored at <-20 ℃ in a manual defrost freezer. The media can be thawed at 2 ℃  to 8 ℃  or at room temperature. Thawed media can be aliquoted and stored at <-20 ℃  in a manual defrost freezer for up to 3 months or used within a month when stored in the dark at 2 ℃  to 8 ℃ . Avoid repeated freeze-thaw cycles.

Precautions

The human origin-derived components used in this product have been derived from human plasma, which has been tested and found negative for antibodies to HIV-1/2, hepatitis B surface antigen (HBsAg), and hepatitis C virus (HCV). However, the medium should be handled as if potentially infectious. Safe laboratory procedures should be followed and protective clothing should be worn when handling this media. The acute and chronic effects of over-exposure to this media are unknown.

Limitations

FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

The safety and efficacy of this product in diagnostic or other clinical uses has not been established.

This reagent should not be used beyond the expiration date indicated on the label.

Results may vary due to variations between dendritic cell precursors/progenitor cells derived from different donors.

Data Examples

Differentiation of CD14+ Monocytes into Dendritic Cells.LPS-matured monocyte-derived dendritic cells were obtained after CD14+-enriched monocytes were cultured for nine days in StemXVivo Serum-Free Dendritic Cell Base Media (Catalog # CCM003) supplemented with GM-CSF (Catalog # 215-GM) and IL-4 (Catalog # 204-IL).

Phenotypic Analysis of Cultured Monocyte-derived Dendritic Cells Before and After LPS-induced Maturation. Immature monocyte-derived dendritic cells (MoDCs) (open histograms; green line) were obtained after CD14+-enriched monocytes were cultured for seven days in StemXVivo庐Serum-Free Dendritic Cell Base Media (Catalog # CCM003) supplemented with 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) and 35 ng/mL Recombinant Human IL-4 (Catalog # 204-IL). Mature monocyte-derived dendritic cells (filled histograms) were cultured under the same conditions for seven days and then induced with LPS for an additional 48 hours. Day seven immature MoDCs and day nine LPS-treated MoDCs were stained with a FITC-conjugated Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # FAB140F), a FITC-conjugated Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # FAB1774F), a FITC-conjugated Mouse Anti-Human B7-2/CD86 Monoclonal Antibody (Catalog # FAB141F), an Anti-MHC Class II Antibody, or an appropriate isotype control antibody (empty histogram with solid line).

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 掳C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Hematopoietic Stem Cells

The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long mainte颅nance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continu颅ously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage deple颅tion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.

Related Research Areas

Hematopoietic Stem Cell Markers

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068