R&D  SC004 Mouse Oligodendrocyte Differentiation Kit (1 KT)

产品编号 SC004 | rndsystems

Mouse Oligodendrocyte Differentiation Kit (1 KT)

产品介绍

Product Details

Kit Summary

A kit optimized to efficiently differentiate mouse pluripotent cells into oligodendrocytes under serum-free conditions.

Key Benefits

Pre-optimized to reduce experimental variation

Defined conditions decrease variation

High lot-to-lot consistency decreases variation

Why Use Pre-optimized Reagents for Differentiation of Pluripotent Stem Cells into Oligodendrocytes?

• Successful differentiation of pluripotent stem cells into oligodendrocytes is dependent on high quality media and differentiation supplements.
• Oligodendrocytes are cells of the central nervous system which myelinate axons and support rapid nerve conduction. In CNS disorders, such as stroke, multiple sclerosis and spinal cord injury, demyelination of axons contributes to functional deficits. Studies have demonstrated that neural transplantation therapy can restore functions lost by demyelination. However, the use of neural transplantation is limited by an inadequate supply of oligodendrocyte precursors. R&D Systems offers the Mouse Oligodendrocyte Differentiation Kit to drive successful and efficient differentiation of embryonic and induced pluripotent stem cells into oligodendrocytes.

R&D Systems Mouse Oligodendrocyte Differentiation Kit:

Is pre-optimized to reduce variation.

Has defined components to decrease variation.

Includes sufficient reagents for the differentiation of 3 x 10⁷ embryonic stem cells.

Kit Components

• The Mouse Oligodendrocyte Differentiation Kit contains ITS and N-2 MAX Media Supplements, which are used to select and enrich neural precursor populations that are characterized by Nestin and A2B5 staining. Purified Bovine Fibronectin is included to support cell attachment and spreading. A growth factor panel, consisting of Recombinant Human Fibroblast Growth Factor Basic (FGF basic), Recombinant Human Epidermal Growth Factor (EGF), and Recombinant Human Platelet-derived Growth Factor AA (PDGF-AA) is included for effective oligodendrocyte differentiation.
• The quantity of each component provided in the kit is estimated to be sufficient for the differentiation of 3 x 10⁷ embryonic stem cells.

Recombinant Human EGF

Recombinant Human FGF basic

Recombinant Human PDGF-AA

Bovine Fibronectin

ITS Media Supplement

N-2 MAX Media Supplement

Stability and Storage

Unopened Kit should be stored at = -20 °C in a manual defrost freezer. Do not use the kit past the expiration date.

Opened Reagents can be stored at 2 °C to 8 °C for up to 1 month or aliquotted and stored at = -20 °C in a manual defrost freezer for up to 6 months.* Avoid repeated freeze-thaw cycles.

• *Provided this is within the expiration date of the kit.

Limitations of the Procedure

FOR LABORATORY RESEARCH USE ONLY. NOT FOR DIAGNOSTIC USE.

The safety and efficacy of this product in diagnostic or other clinical uses has not been established.

The quality of the embryonic stem cells and any variation in this procedure may cause variation in the efficiency of oligodendrocyte generation.

Precautions

The acute and chronic effects of overexposure to reagents of this kit are unknown. Safe laboratory procedures should be followed and protective clothing should be worn when handling kit reagents.

The ITS and N-2 MAX Supplements contain human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection.

• Data Examples
• Mouse Oligodendrocytes Generated Using the Mouse Oligodendrocyte Differentiation Kit. D3 mouse embryonic stem cells were expanded in KO-ES Media and differentiated into oligodendrocytes using the Mouse Oligodendrocyte Differentiation Kit (Catalog # SC004). Oligodendrocytes were detected using a Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326). The cells were stained with the NorthernLights™-557 Affinity-purified Goat Anti-Mouse Secondary Antibody (Catalog # NL019). The nuclei were counterstained with DAPI. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
• Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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