R&D  SC001B Human/Mouse Dopaminergic Neuron Differentiation Kit (1 KT)

产品编号 SC001B| rndsystems

Human/Mouse Dopaminergic Neuron Differentiation Kit (1 KT)

产品介绍

Product Details

Kit Summary

A kit optimized to efficiently differentiate pluripotent cells into dopaminergic neurons under serum-free conditions.

Key Benefits

Pre-optimized kit reduces experimental variation

Defined conditions decreases variation

Includes a panel of premium quality growth factors

Why Use Pre-optimized Reagents to Differentiate Pluripotent Stem Cells into Dopaminergic Neurons?

• The loss of dopaminergic neurons in the substantia nigra is a characteristic of Parkinson’s disease (PD), a neurodegenerative disorder that affects motor function.
• Both animal models and clinical trials have suggested that cell replacement therapies may be effective in treating PD. However, this approach is limited by the availability of a rich, effective source of neural precursors for dopaminergic neuron generation. While embryonic and induced pluripotent stem cells can be differentiated into dopaminergic neurons, successful differentiation is dependent on high quality media and defined differentiation supplements. R&D Systems offers the Human/Mouse Dopaminergic Neuron Differentiation Kit to drive successful and efficient differentiation of embryonic and induced pluripotent stem cells into dopaminergic neurons.

R&D Systems Human/Mouse Dopaminergic Neuron Differentiation Kit:

Includes high quality reagents to generate dopaminergic neurons in 27 days.

Includes pre-optimized components with low lot-to-lot variation.

Includes sufficient reagents for differentiation of 3 x 10⁷ pluripotent stem cells.

Kit Components

The Human/Mouse Dopaminergic Neuron Differentiation Kit contains ITS and N-2 MAX Media Supplements, which are used to select and enrich neural stem cell populations. Bovine Fibronectin is included to support cell attachment and spreading. A growth factor panel, consisting of Recombinant Human Fibroblast Growth Factor Basic (FGF basic), Recombinant Mouse Fibroblast Growth Factor 8b (FGF-8b) and Recombinant Mouse Sonic Hedgehog Amino-terminal Peptide (Shh-N), is included for effective dopaminergic differentiation.

The quantity of each component provided in the kit is estimated to be sufficient for differentiation of 3 x 10⁷ embryonic stem cells.

Recombinant Mouse FGF-8b

Recombinant Human FGF basic

Recombinant Mouse Shh-N

Purified Bovine Fibronectin

ITS Media Supplement

N-2 MAX Media Supplement

• Data Examples
• Characterization of Dopaminergic Neurons Generated from Human Pluripotent Stem Cells. Dopaminergic neurons were generated from human pluripotent stem cells using the Dopaminergic Neuron Differentiation Kit (Catalog # SC001B). Tyrosine Hydroxylase was detected using a Mouse Anti-Human Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566). The cells were stained with the NorthernLights™ (NL) 557-conjugated Donkey Anti-Mouse Antigen Affinity-purified Secondary Antibody (Catalog # NL007, red). Neuron-specific beta-III Tubulin was detected using a Mouse Anti-Neuron-specific beta-III Tubulin (Clone Tuj-1) Monoclonal Antibody (Catalog # MAB1195) followed by the NL493-flourochrome-conjugated Donkey Anti-Mouse Antigen Affinity-purified Secondary Antibody (Catalog # NL009, green). View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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