R&D  AR012 N21-MAX Vitamin A Free Media Supplement (50X)

产品编号 AR012 | rndsystems

N21-MAX Vitamin A Free Media Supplement (50X)

产品介绍

Product Details

Kit Summary

For controlled neural stem and progenitor cell expansion.

Key Benefits

Optimized for neural stem cell expansion

Limits retinoic acid production to reduce unwanted NSC differentiation

Rigorous quality control testing ensures consistency across experiments

Eliminates the need for an astrocyte feeder layer

Is nutrient-rich, serum-free, and fully defined

Why Culture Neurons in Serum-Free N21-MAX Media Supplement?

• The N21-MAX Vitamin A Media Supplement is a serum-free and fully-defined media formulation that is optimized for the consistent health and superior growth of neural stem/progenitor cells. The N21-MAX Vitamin A Free Supplement limits NSC differentiation by reducing unwanted retinoic acid production in cell culture medium. It can also be used to mature and maintain healthy primary neurons in culture.
• N21-MAX media supplements were designed to eliminate uncontrolled variables, such as those found in both serum-containing media and in some commercial serum-free neuronal supplements. Use of undefined media supplements can confound neuronal growth and maturation. N21-MAX media supplements contain a cocktail of factors optimized for neural stem cell expansion, neuronal survival, and synaptic maturation. Each lot of N21-MAX Media Supplement is tested on primary hippocampal neurons to ensure consistent performance for the user.
• The N21-MAX Vitamin A Free Media Supplement:

Is fully defined and quality tested to reduce unwanted experimental variation.

Contains factors qualified to the expansion of NSCs in vitro.

Limits retinoic acid production to reduce unwanted NSC differentiation.

Does not require an astrocyte feeder layer.

N21-MAX Vitamin A Free Media Supplement Components

• N21-MAX Vitamin A-Free Media Supplement is optimized for the expansion of neural stem cells in culture. The supplement is supplied as a 50X concentrated solution and contains the following 19 components:

Albumin (bovine)

L-Carnitine

Catalase

Corticosterone

Ethanolamine

Glutathione

Galactose

Holo-Transferrin

Insulin

Linoleic Acid

Linolenic Acid

Lipoic Acid

Progesterone

Putrescine

Selenite

Superoxide dismutase

Triiodo-L-thyronine

D,L-alpha-Tocopherol

D,L-alpha-Tocopherol acetate

• Supplied in a volume sufficient to supplement 500 mL of media at the recommended concentration. N21-MAX Vitamin A Free Media Supplement is tested for use in Neurobasal Media.

Precautions

• This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection.
• Data Examples
• Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Vitamin A Free Media Supplement. E18 rat hippocampal neurons grown for 21 days in vitro in media supplemented with N21-MAX Vitamin A Free Media Supplement. The cells were stained with Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364) followed by the NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue).

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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