R&D  AR010 N21-MAX Insulin Free Media Supplement (50X)

产品编号 AR010| rndsystems

N21-MAX Insulin Free Media Supplement (50X)

产品介绍

Product Details

Kit Summary

For the reliable maturation and superior long-term maintenance of primary neurons under insulin free culture conditions.

Key Benefits

Ideal for studies of insulin secretion or insulin receptor function

Optimized formulation of the original B27 recipe

Rigorous quality control testing ensures consistency across experiments

Eliminates the need for an astrocyte feeder layer

Is nutrient-rich, serum-free, and fully defined

Why Culture Neurons in Serum-Free N21-MAX Media Supplement?

• The N21-MAX Insulin Free Media Supplement improves upon the traditional B27¹ and NS21² neuronal supplements, offering a serum-free, insulin-free, and fully-defined formulation that is optimized for the reliable maturation, consistent health, and superior function of neurons in culture. This insulin-free formulation is ideal insulin-sensitive research, including studies focused on insulin secretion or insulin receptor function.   See Details

N21-MAX Insulin Free Media Supplement Components

• N21-MAX Insulin Free Media Supplement is optimized for the maturation and long-term culturing of neuronal cells. The supplement is supplied as a 50X concentrated solution and contains the following 20 components:  See Details

Precautions

• This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection.
• Data Examples
• Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Insulin Free Media Supplement.E18 rat hippocampal neurons were grown for 21 days in vitro in media supplemented with N21-MAX Insulin Free Media Supplement. The cells were stained with the Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (R&D Systems, Catalog # MAB4364), followed by the NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; R&D Systems, Catalog # NL007). The nuclei were counterstained with DAPI.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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