R&D  CCM021  StemXVivo Xeno-Free Human MSC Expansion Media

产品编号 CCM021 | rndsystems

StemXVivo Xeno-Free Human MSC Expansion Media

Media for Human Mesenchymal Stem Cell Expansion

产品介绍

Kit Summary

Xeno-free media optimized for the expansion of human mesenchymal stem cells.

Key Benefits

Maintains MSCs in an undifferentiated state

Free of animal components that can introduce variability and pose a safety risk

Suitable for translational studies

Optimized for efficient expansion of human MSCs

Why use Xeno-Free Media to Expand Mesenchymal Stem Cells?

• Mesenchymal stem cells are often expanded in media that contain serum and other components that are of animal origin. The presence of animal-derived factors in media can introduce experimental variability and limit the use of the cultured cells in translational or clinical studies.
• R&D Systems offers StemXVivo Xeno-Free Human MSC Expansion Media, a media that is optimized for efficient expansion of human MSCs in the absence of animal-derived components. MSCs expanded in StemXVivo Xeno-Free Human MSC Expansion Media maintain a characteristic fibroblast-like morphology, remain in an undifferentiated state, and express MSC markers.

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

• The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’   See Details

StemXVivo Xeno-Free Human MSC Expansion Media Components

• Supplied in a 500 mL volume, StemXVivo Xeno-Free Human MSC Media contains high quality factors to support MSC expansion in vitro. This media does not contain antibiotics.

Stability and Storage

• StemXVivo Xeno-Free Human MSC Expansion Media is stable until the expiration date when stored at -20 °C in a manual defrost freezer. The media should be thawed at 2 °C to 8 °C. Thawed media can be aliquoted and stored at -20 °C in a manual defrost freezer for up to 3 months or used within one month when stored in the dark at 2 °C to 8 °C. Avoid repeated freeze-thaw cycles.

Data Examples

• Morphology of Mesenchymal Stem Cells (MSCs) Cultured in StemXVivo Xeno-Free Human MSC Expansion Media. Bone marrow-derived human MSCs were cultured in StemXVivo Xeno-Free Human MSC Expansion Media (Catalog # CCM021) for 4 passages and then imaged using brightfield microscopy.
• Expression of Mesenchymal Stem Cell (MSC) Markers in MSCs Expanded in R&D Systems StemXVivo Xeno-Free Human MSC Expansion Media or a Competitor’s Xeno-Free MSC Expansion Media. Human bone marrow-derived MSCs were expanded for 4 passages in StemXVivo Xeno-Free Human MSC Expansion Media (Catalog # CCM021) or a competitor’s xeno-free MSC expansion media. The cells were harvested and stained for the expression of positive and negative MSC markers. Positive MSC markers were detected with APC-conjugated Mouse Anti-Human Endoglin/CD105 Monoclonal Antibody (Catalog # FAB10971A), PE-conjugated Mouse Anti-Human 5’-Nucleotidase/CD73 Monoclonal Antibody (Catalog # FAB5795P), and APC-conjugated Mouse Anti-Human CD90/Thy1 Monoclonal Antibody (Catalog # FAB2067A). Expression of the negative MSC marker, CD45 was detected with PE-conjugated Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # FAB1430P). Quadrants were set based on isotype controls.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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