R&D  CCM020  StemXVivo Rat Chondrogenic Supplement, 0.5 mL

产品编号 CCM020 | rndsystems

StemXVivo Rat Chondrogenic Supplement, 0.5 mL

产品介绍

Product Details

Kit Summary

Media supplement to induce the differentiation of rat MSCs into chondrocytes. For use with Human/Mouse/Rat StemXVivo Chondrogenic Base Media (Catalog # CCM005).

Key Benefits

Supports induction of chondrogenesis in rat MSCs

Defined supplement to reduce experimental variation

Developed and optimized using rat MSCs

Why Induce Chondrogenesis in MSCs with a Defined Media Supplement?

• Despite the well-characterized factors and protocols used to differentiate mesenchymal stem/stromal cells (MSCs) into chondrocytes, differentiation efficiencies can vary depending on the quality of the MSC starting population and the reagents used to expand and differentiate MSCs.  

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

• The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’

Rat StemXVivo Chondrogenic Supplement Components

• This media supplement contains defined, high quality factors to drive rat MSC differentiation into chondrocytes.  

Data Examples

• Detection of Aggrecan in a Rat MSC-differentiated Chondrogenic Pellet Section. Rat MSCs were cultured for 21 days using the Human/Mouse StemXVivo Chondrogenic Base Media (Catalog # CCM005) and Rat StemXVivo Chondrogenic Supplement (Catalog # CCM020) and the resulting chondrogenic pellet was cryosectioned. Chondrocyte differentiation was verified using a Goat Anti-Human Aggrecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220). The cells were stained using a NorthernLights™ 557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # NL001; red) and the nuclei were counterstained with DAPI (blue).
• MSCs Differentiated into Chondrocytes form Characteristic Cell Pellets. Human MSCs cultured with StemXVivo Chondrogenic Base Media (Catalog # CCM005) and StemXVivo Chondrogenic Supplement (Catalog # CCM006) formed a chondrogenic pellet (ball) imaged here at day 21 of culture. Rat MSCs also form chondrogenic pellets when cultured in Chondrogenic Supplement (data not shown).

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068