R&D  CCM016  StemXVivo Serum-Free MSC Freezing Media

产品编号 CCM016 | rndsystems

StemXVivo Serum-Free MSC Freezing Media

For serum-free cryopreservation of Human/Mouse/Rat Mesenchymal Stem Cells (MSCs)

产品介绍

Product Details

Kit Summary

Freezing media for human, mouse, or rat MSC cryopreservation.

Key Benefits

All components were selected and optimized for MSC cryopreservation

Ready to use

Recover up to 50% more viable MSCs compared to conventional cryopreservatives

Why use a Defined Media for MSC Cryopreservation?

• Mesenchymal stem/stromal cells (MSCs) are a rare population of bone marrow-derived cells. Given their rarity, MSCs are most often expanded in vitro under specific culture conditions.   See Details

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

• The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’   See Details

StemXVivo MSC Freezing Media Components

• Supplied in a 50 mL volume, this media contains defined, high quality factors to support MSC cryopreservation.

Does not contain antibiotics.

Contains 10% dimethylsulfoxide (DMSO).

Data Examples

• MSC Viability Following Cryopreservation in StemXVivo Serum-Free MSC Freezing Media. Human MSCs were frozen in either conventional, serum-containing freezing media or StemXVivo Serum-Free MSC Freezing Media (Catalog # CCM016) at 2 x 10⁵cells/cryovial and stored in liquid nitrogen for one week. The MSCs were thawed, resuspended in StemXVivo Serum-Free Human MSC Expansion Media (Catalog # CCM014), and cultured in 6-well plates coated with Recombinant Human Fibronectin (Catalog # 4305-FN). After one day in culture, the MSCs were harvested in triplicate, stained with Trypan blue, and viable cells were counted using a hemocytometer. A greater percentage of viable MSCs were recovered following cryopreservation in StemXVivo MSC Freezing Media compared to conventional, serum-containing freezing media.
• MSC Identification Following Cryopreservation in StemXVivo Serum-Free MSC Freezing Media. Human MSCs were frozen in StemXVivo Serum-Free MSC Freezing Media (Catalog # CCM016) at 2 x 10⁵ cells/cryovial and stored in liquid nitrogen for one week. The MSCs were thawed, resuspended in StemXVivo Serum-Free Human MSC Expansion Media (Catalog # CCM014), and cultured in 6-well plates coated with Recombinant Human Fibronectin (Catalog # 4305–FN). After one day in culture, the MSCs were harvested and incubated with a PE-conjugated Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # FAB1430P; filled histogram) and an APC-conjugated Mouse Anti-Human CD90/Thy1 Monoclonal Antibody (Catalog # FAB2067A; filled histogram), or a Mouse IgG₁or IgG_2A Isotype Control (Catalog # IC002P, or IC003A, respectively; open histograms). Thawed MSCs were positive for the MSC multipotency marker, CD90/Thy1 and negative for the hematopoietic marker, CD45.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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