R&D  CCM014  StemXVivo Serum-Free Human MSC Expansion Media

产品编号 CCM014 | rndsystems

StemXVivo Serum-Free Human MSC Expansion Media

Mesenchymal Stem Cell Expansion

产品介绍

Product Details

Kit Summary

A complete media formulated and optimized for the maintenance and expansion of purified human MSCs.

Key Benefits

Defined medium reduces experimental variation

All components were selected and optimized for human MSCs

Ready to use

Why Culture Human MSCs in a Defined, Serum-Free Medium?

• Mesenchymal stem/stromal cells (MSCs) are a rare population of multipotent cells that can be derived from a number of tissues such as bone marrow, adipose, and placenta.   See Details

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

• The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’   See Details

StemXVivo MSC Expansion Media Components

• Supplied in a 500 mL volume, this medium contains high quality factors to support MSC expansion in vitro.  

Data Examples

• Morphology of Human MSCs. Human MSCs were cultured with StemXVivo Serum-Free Human MSC Expansion Media (Catalog # CCM014) on plates coated with Recombinant Human Fibronectin (Catalog # 4305-FN). Characteristic cell morphology was observed at 90% confluency.
• Phenotypic Analysis of Human MSCs Expanded in StemXVivo Serum-Free Human Mesenchymal Stem Cell Expansion Media for 3 Passages. Human MSCs were stained with the following Anti-Human Antibodies to verify MSC identity: CD105 (Catalog # FAB10971P), CD90 (Catalog # FAB2067P), CD73 (Catalog # FAB5795P), CD44 (Catalog # FAB4948P), CD45 (Catalog # FAB1430P), CD11b (Catalog # FAB16991P), and CD34 (filled histograms). Cells were analyzed using a Becton Dickinson FACSCalibur™ flow cytometer. For each antibody, isotype-matched controls are shown with an open histogram.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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