R&D  CCM006 StemXVivo Human/Mouse Chondrogenic Supplement, 0.5 mL (1 VL)

产品编号 CCM006 | rndsystems

StemXVivo Human/Mouse Chondrogenic Supplement, 0.5 mL

产品介绍

Product Details

Kit Summary

Base media for the differentiation of MSCs into chondrocytes. For use with Human/Mouse and Rat StemXVivo^® Chondrogenic Supplements.

Key Benefits

Supports induction of chondrogenesis in MSCs

Defined supplement reduces experimental variation

Developed and optimized using MSCs

Why Induce Chondrogenesis in MSCs with a Defined Media Supplement?

• Despite the well-characterized factors and protocols used to differentiate mesenchymal stem/stromal cells (MSCs) into chondrocytes, differentiation efficiencies can vary depending on the quality of the MSC starting population and the reagents used to expand and differentiate MSCs.

Human/Mouse StemXVivo^® Chondrogenic Supplement:

Contains high quality differentiation factors to drive reproducible and efficient MSC chondrogenesis.

Is defined to reduce unwanted experimental variability.

Has been developed and optimized using human and mouse MSCs.

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

• The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’
• Mesenchymal stem cells are multipotent, self-renewing cells that have the ability to differentiate into adipocytes, chondrocytes, and osteoblasts when cultured in vitro. Read More about MSC Nomenclature

Human StemXVivo^® Osteogenic Supplement Components

• This media supplement contains high quality factors to drive human MSC differentiation into osteocytes.

This supplement requires media (not included), such as Human/Mouse/Rat StemXVivo^®Osteogenic/Adipogenic Base Media (Catalog # CCM007) or equivalent.

The quantity of osteogenic media supplement supplied is sufficient to make 250 mL of media for differentiation.

Data Examples

MSCs Differentiated into Chondrocytes form Characteristic Cell Pellets. Human MSCs cultured with Human/Mouse/Rat StemXVivo^® Chondrogenic Base Media (Catalog # CCM005) and Human/Mouse StemXVivo^® Chondrogenic Supplement (Catalog # CCM006) formed a chondrogenic pellet (ball) imaged here at day 21 of culture.

Detection of Aggrecan in a Human MSC-differentiated Chondrogenic Pellet Section. Human MSCs cultured with Human/Mouse/Rat StemXVivo^® Chondrogenic Base Media (Catalog # CCM005) and Human/Mouse StemXVivo^®Chondrogenic Supplement (Catalog # CCM006) and the resulting chondrogenic pellet was cryosectioned. Chondrocyte differentiation was verified using a Goat Anti-Human Aggrecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220). The cells were stained using a NorthernLights^™ 557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # NL001) and the nuclei were counterstained with DAPI.

Detection of Collagen II in a Mouse MSC-differentiated Chondrogenic Pellet Section. Mouse MSCs were cultured for 21 days using the Human/Mouse StemXVivo^® Chondrogenic Base Media (Catalog # CCM005) and Human/Mouse StemXVivo^®Chondrogenic Supplement (Catalog # CCM006) and the resulting chondrogenic pellet was cryosectioned. Chondrocyte differentiation was verified using a Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615). The cells were stained using a NorthernLights^™ 557-conjugated Donkey Anti-Sheep Secondary Antibody (Catalog # NL010).

2006 Proposed Change to MSC Nomenclature

• Although mesenchymal stromal cells were once referred to as ‘mesenchymal stem cells’, a change to ‘mesenchymal stromal cells’ was proposed by the International Society for Cellular Therapy in 2006.¹
• The change in nomenclature originates from two important factors:

Methods used to isolate mesenchymal stem cells yield a heterogeneous population of cells with only a fraction of these cells demonstrating multipotency.

The absence of direct evidence that mesenchymal stem cells can self-renew and differentiate in vivo.

Use of Mesenchymal Stem and Stromal Cell Terminology

• Data supporting MSC self-renewal and multipotency have been obtained using in vitro conditions, which does not adequately reflect the in vivo environment. The lack of in vivo data has led some researchers to question the validity of the term ‘mesenchymal stem cell’ providing further support for the use of ‘mesenchymal stromal cells’ to describe MSCs.² While ‘mesenchymal stromal cells’ may be the more scientifically accurate term for MSCs, the two terms are often used interchangeably in the literature. R&D Systems recognizes the use of both mesenchymal stem cells and mesenchymal stromal cells and uses ‘MSC’ to indicate mesenchymal stem/stromal cells to account for both designations.

Definitions of Mesenchymal Stromal Cells and Mesenchymal Stem Cells

Mesenchymal Stromal Cells – A heterogeneous population of cultured cells with similar characteristics such as the ability to adhere to plastic and the expression of specific marker proteins.

Mesenchymal Stem Cells – A subpopulation of mesenchymal stromal cells that have the capacity to self-renew and differentiate into mesodermal lineages when cultured in vitro. The capacity to self-renew and differentiate in vivo has yet to be clearly demonstrated for mesenchymal stem cells.

References

Dominici, M. et al. (2006) Cytotherapy 8:315.

Keating, A. (2012) Cell Stem Cell 10:709.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

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