R&D  CCM004 StemXVivo Mesenchymal Stem Cell Expansion Media

产品编号 CCM004 | rndsystems

StemXVivo Mesenchymal Stem Cell Expansion Media

For use with human, mouse, and rat Mesenchymal Stem Cells (MSCs)

产品介绍

Product Details

Kit Summary

A complete, fetal bovine serum-containing media formulated and optimized for the maintenance and expansion of purified human, mouse, and rat MSCs.

Key Benefits

Formulated to reduce variation

All components, including fetal bovine serum, were selected and optimized for MSCs

Ready to use

Why Culture MSCs in a Defined Medium?

• Mesenchymal stem/stromal cells (MSCs) are a rare population of multipotent cells that can be derived from a number of tissues such as bone marrow, adipose, and placenta.
• Given their rarity and sensitivity to ex vivo conditions, MSCs are most often expanded in vitro under specific culture conditions.

StemXVivo^® MSC Expansion Media:

Supports reproducible MSC growth in vitro.

Can be supplemented with user-defined cytokines and growth factors, although these are not needed for culture

Has been developed and optimized for use with human, mouse, and rat MSCs.

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

• The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’
• Mesenchymal stem cells are multipotent, self-renewing cells that have the ability to differentiate into adipocytes, chondrocytes, and osteoblasts when cultured in vitro. Read More about MSC Nomenclature

Human StemXVivo^® Osteogenic Supplement Components

• This media supplement contains high quality factors to drive human MSC differentiation into osteocytes.

This supplement requires media (not included), such as Human/Mouse/Rat StemXVivo^®Osteogenic/Adipogenic Base Media (Catalog # CCM007) or equivalent.

The quantity of osteogenic media supplement supplied is sufficient to make 250 mL of media for differentiation.

Data Examples

Phenotypic Analysis of Human MSCs Expanded in StemXVivo^® MSC Expansion Media. Human MSCs were expanded using StemXVivo^® Mesenchymal Stem Cell (MSC) Expansion Media (Catalog # CCM004). Filled histograms indicate cells stained with markers of undifferentiated MSCs including anti-CD105 (Catalog # FAB10971P) or CD45 (Catalog # MAB1430). The open histograms show isotype-matched control staining. MSCs appropriately lack expression of CD45.




Phenotypic Analysis of Mouse MSCs Expanded in StemXVivo^® MSC Expansion Media. Mouse MSCs were expanded using StemXVivo^® Mesenchymal Stem Cell (MSC) Expansion Media (Catalog # CCM004). Filled histograms indicate cells stained with markers of undifferentiated MSCs including anti-CD44 (Catalog # AF6127) or CD45 (Catalog # MAB114). The open histograms show isotype-matched control staining. MSCs appropriately lack expression of CD45.




Phenotypic Analysis of Rat MSCs Expanded in StemXVivo^®MSC Expansion Media. Rat MSCs were expanded using StemXVivo^® Mesenchymal Stem Cell (MSC) Expansion Media (Catalog # CCM004). Filled histograms indicate cells stained with markers of undifferentiated MSCs including anti-CD90 or CD45. The open histograms show isotype-matched control staining. MSCs appropriately lack expression of CD45.

2006 Proposed Change to MSC Nomenclature

• Although mesenchymal stromal cells were once referred to as ‘mesenchymal stem cells’, a change to ‘mesenchymal stromal cells’ was proposed by the International Society for Cellular Therapy in 2006.¹
• The change in nomenclature originates from two important factors:

Methods used to isolate mesenchymal stem cells yield a heterogeneous population of cells with only a fraction of these cells demonstrating multipotency.

The absence of direct evidence that mesenchymal stem cells can self-renew and differentiate in vivo.

Use of Mesenchymal Stem and Stromal Cell Terminology

• Data supporting MSC self-renewal and multipotency have been obtained using in vitro conditions, which does not adequately reflect the in vivo environment. The lack of in vivo data has led some researchers to question the validity of the term ‘mesenchymal stem cell’ providing further support for the use of ‘mesenchymal stromal cells’ to describe MSCs.² While ‘mesenchymal stromal cells’ may be the more scientifically accurate term for MSCs, the two terms are often used interchangeably in the literature. R&D Systems recognizes the use of both mesenchymal stem cells and mesenchymal stromal cells and uses ‘MSC’ to indicate mesenchymal stem/stromal cells to account for both designations.

Definitions of Mesenchymal Stromal Cells and Mesenchymal Stem Cells

Mesenchymal Stromal Cells – A heterogeneous population of cultured cells with similar characteristics such as the ability to adhere to plastic and the expression of specific marker proteins.

Mesenchymal Stem Cells – A subpopulation of mesenchymal stromal cells that have the capacity to self-renew and differentiate into mesodermal lineages when cultured in vitro. The capacity to self-renew and differentiate in vivo has yet to be clearly demonstrated for mesenchymal stem cells.

References

Dominici, M. et al. (2006) Cytotherapy 8:315.

Keating, A. (2012) Cell Stem Cell 10:709.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068