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R&D  CCM005  StemXVivo Chondrogenic Base Media

产品编号 CCM005| rndsystems

StemXVivo Chondrogenic Base Media

产品介绍

Kit Summary

Base media for the differentiation of MSCs into chondrocytes. For use with Human/Mouse and Rat StemXVivo  Chondrogenic Supplements.

Key Benefits

Defined formulation that reduces experimental variation

Supports induction of chondrogenesis in MSCs

Developed and optimized using MSCs

Why Induce Chondrogenesis in MSCs with Defined Media?

Despite the well-characterized factors and protocols used to differentiate mesenchymal stem/stromal cells (MSCs) into chondrocytes, differentiation efficiencies can vary depending on the quality of the MSC starting population and the reagents used to expand and differentiate MSCs.

StemXVivo® Chondrogenic Base Media:

Is defined to support reproducible MSC chondrogenesis.

Offers flexibility to evaluate novel cytokine and growth factor combinations to induce chondrogenesis.

Has been developed and optimized using MSCs.

Can be used with StemXVivo® Human/Mouse or Rat Chondrogenic Supplements to reduce variation during chondrogenesis.

Mesenchymal Stromal Cells or Mesenchymal S tem Cells?

The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’

Mesenchymal stem cells are multipotent, self-renewing cells that have the ability to differentiate into adipocytes, chondrocytes, and osteoblasts when cultured in vitro. Read More about MSC Nomenclature

Human/Mouse/Rat StemXVivo® Chondrogenic Base Media

Supplied in a 50 mL volume, this media contains high quality factors to drive MSC differentiation into chondrocytes when used with additional differentiation factors.

Supplemented with sodium bicarbonate but does not contain antibiotics.

*This medium requires supplements (not included), such as Human/Mouse StemXVivo® Chondrogenic Supplement (Catalog # CCM006), Rat StemXVivo® Chondrogenic Supplement (Catalog # CCM020), or user-defined cytokines and growth factors to induce chondrogenesis.

Data Examples

MSCs Differentiated into Chondrocytes form Characteristic Cell Pellets. Human MSCs cultured with StemXVivo Chondrogenic Base Media (Catalog # CCM005) and StemXVivo Chondrogenic Supplement (Catalog # CCM006) formed a chondrogenic pellet (ball) imaged here at day 21 of culture.

Detection of Aggrecan in a Human MSC-differentiated Chondrogenic Pellet Section. Human MSCs were cultured with StemXVivo® Chondrogenic Base Media (Catalog # CCM005) and StemXVivo® Chondrogenic Supplement (Catalog # CCM006) and the resulting chondrogenic pellet was cryosectioned. Chondrocyte differentiation was verified using a Goat Anti-Human Aggrecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220). The cells were stained using a NorthernLights™ 557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # NL001; red) and the nuclei were counterstained with DAPI (blue).

Detection of Collagen II in a Mouse MSC-differentiated Chondrogenic Pellet Section. Mouse MSCs were cultured for 21 days using the Human/Mouse StemXVivo® Chondrogenic Base Media (Catalog # ;CCM005) and Human/Mouse StemXVivo® Chondrogenic Supplement (Catalog # CCM006) and the resulting chondrogenic pellet was cryosectioned. Chondrocyte differentiation was verified using a Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615). The cells were stained using a NorthernLights™ 557-conjugated Donkey Anti-Sheep Secondary Antibody (Catalog # NL010; red) and the nuclei were counterstained with DAPI (blue).

2006 Proposed Change to MSC Nomenclature

Although mesenchymal stromal cells were once referred to as ‘mesenchymal stem cells’, a change to ‘mesenchymal stromal cells’ was proposed by the International Society for Cellular Therapy in 2006.1

The change in nomenclature originates from two important factors:

Methods used to isolate mesenchymal stem cells yield a heterogeneous population of cells with only a fraction of these cells demonstrating multipotency.

The absence of direct evidence that mesenchymal stem cells can self-renew and differentiate in vivo.

Use of Mesenchymal Stem and Stromal Cell Terminology

Data supporting MSC self-renewal and multipotency have been obtained using in vitro conditions, which does not adequately reflect the in vivo environment. The lack of in vivo data has led some researchers to question the validity of the term ‘mesenchymal stem cell’ providing further support for the use of ‘mesenchymal stromal cells’ to describe MSCs.2 While ‘mesenchymal stromal cells’ may be the more scientifically accurate term for MSCs, the two terms are often used interchangeably in the literature. R&D Systems recognizes the use of both mesenchymal stem cells and mesenchymal stromal cells and uses ‘MSC’ to indicate mesenchymal stem/stromal cells to account for both designations.

Definitions of Mesenchymal Stromal Cells and Mesenchymal Stem Cells

Mesenchymal Stromal Cells – A heterogeneous population of cultured cells with similar characteristics such as the ability to adhere to plastic and the expression of specific marker proteins.

Mesenchymal Stem Cells – A subpopulation of mesenchymal stromal cells that have the capacity to self-renew and differentiate into mesodermal lineages when cultured in vitro. The capacity to self-renew and differentiate in vivo has yet to be clearly demonstrated for mesenchymal stem cells.

References

Dominici, M. et al. (2006) Cytotherapy 8:315.

Keating, A. (2012) Cell Stem Cell 10:709.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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