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R&D  SC030B  StemXVivo Mesoderm Kit

产品编号 SC030B| rndsystems

StemXVivo Mesoderm Kit

For mesoderm differentiation of human pluripotent stem cells

产品介绍

Kit Summary

For the differentiation of human pluripotent stem cells into mesoderm.

Key Benefits

Mesoderm differentiation in 3 days

Optimized components to reduce experimental variation

Contains differentiation supplements and an antibody to verify mesoderm differentiation

Involves validated and straightforward protocols

Why Differentiate Human Pluripotent Stem Cells into Mesoderm In Vitro?

Mesoderm is one of three primary germ layers formed during the process of gastrulation in the vertebrate embryo. Embryonic mesoderm gives rise to several important organs and tissues including the heart, muscle, bone, and cartilage.

Mesoderm differentiation is also an essential first step for the formation of other downstream derivative cell types, which can be used in regenerative medicine as well as toxicological and developmental studies.

The StemXVivo Mesoderm Kit:

Uses fully defined supplements to drive reproducible differentiation.

Produces a healthy mesodermal cell population to increase consistency between studies and reduce unwanted experimental variability.

Provides results in 3 days to save time and reagents.

Kit Contents

This kit contains the following reagents to drive pluripotent stem cell differentiation into mesodermal cells and an antibody to verify differentiation status:

Mesoderm Base Media Supplement (50x)

Mesoderm Differentiation Supplement

Mesoderm Marker: Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody

The quantity of each component in this kit is sufficient to make 100 mL of media for differentiation. This is enough media for the differentiation of six 60 mm plates or two 24-well plates.

Data Examples

Differentiation of Pluripotent Stem Cells into Mesoderm.BG01V human embryonic stem cells were differentiated into mesoderm using the media supplements included in this kit. To evaluate lineage commitment, the cells were stained with the Goat Anti-Human Brachyury Antibody included. The cells were stained using the NorthernLights 557-conjugated Donkey anti-Goat IgG Secondary Antibody (Catalog # NL001; red), and the nuclei were counterstained with DAPI (blue).

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Pluripotent Stem Cells

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitroand still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.

R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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