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R&D AR011 StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)
产品编号 AR011| rndsystems
StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)
产品介绍
Kit Summary
A serum-free cell culture media supplement for the extended culture of cardiomyocytes.
Key Benefits
Optimized for the extended culture of pluripotent stem cell-derived cardiomyocytes
Maintains a healthy cardiomyocyte population for longer than 200 days
Is nutrient-rich, fully defined, and serum-free
High lot-to-lot consistency decreases experimental variation
Why Culture Cardiomyocytes in a Defined Medium?
Maintaining the health of pluripotent stem cell-derived cardiomyocytes is imperative for generating trustworthy data during downstream experimentation. Culturing cardiomyocytes in serum-free, defined media reduces experimental variability in cell health by eliminating the uncontrolled variables that are often associated with serum-containing media, such as indeterminate levels of vitamins, hormones, and growth factors.
Cardiomyocytes cultured using the Cardiomyocyte Maintenance Media Supplement can also be a part of drug toxicity and small molecule screening workflows where a controlled cell culture environment is essential.
Cardiomyocyte Maintenance Media Use
Human pluripotent stem cells are differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (R&D Systems, Catalog # SC032) until widespread beating is observed in the culture. StemXVivo Cardiomyocyte Maintenance Media can then be used to maintain the differentiated cells for extended culture. Media should be replaced daily.
Note: Repeated mechanical stress can negatively affect cell attachment and survival. For extended culture, daily media exchanges should be handled as gently as possible.
Precautions
This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection.
Data Examples
Detection of Cardiac Troponin T in Human ES-derived Cardiomyocytes. BG01V human embryonic stem cells were differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # SC032) and maintained in StemXVivo Cardiomyocyte Maintenance Media Supplement (Catalog # AR011) until day 80 of differentiation. Cardiac Troponin T (red) was detected in cardiomyocytes with a Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) and a NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse Affinity Purified Polyclonal Antibody (Catalog #NL007). The nuclei were counterstained with DAPI (blue).
Troponin I Expression in Human ES-derived Cardiomyocytes.BG01V human embryonic stem cells were differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # SC032) and maintained in StemXVivo Cardiomyocyte Maintenance Media Supplement (Catalog # AR011). Troponin I (red) expression was detected in cardiomyocytes at day 80 of differentiation. The nuclei were counterstained with DAPI (blue). |
Preparation and Storage
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Embryonic Stem Cells
Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitroand still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.
R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.
公司简介
R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。
目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。
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