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InvivoGen  pfuse-hg1lc   pFUSE-Lucia-hG1-Fc

Production of Lucia luciferase-Tagged IgG1-Fc fusion proteins

pFUSE-Lucia-hG1-Fc plasmid allows the production of Lucia luciferase-Tagged IgG1-Fc fusion proteins. This plasmid can be used to make recombinant Lucia luciferase-Tagged IgG1-Fc fusion proteins.

Lucia is a secreted luciferase reporter protein with advantageous characteristics when associated with Fc-fusion proteins. It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.

Quantification of Lucia-Fc expression can be determined using QUANTI-Luc™.

产品描述

pFUSE-Lucia-Fc

pFUSE-Lucia-Fc plasmids allow the production of Lucia luciferase-Tagged Fc-Fusion Proteins. Lucia is a secreted luciferase reporter protein with advantageous characteristics when associated with Fc-fusion proteins. It possesses superior carrier ability for excellent secretion of the chimeric protein.

It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.

Quantification of Lucia-Fc expression can be determined using QUANTI-Luc™.

其他产品线

1-Cell Culture and Transfection Reagents:

Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;

2-Mammalian Expression Vectors:

A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;

3-Reporter Cells:

A growing collection of functionally validated and mycoplasma-free human and murine cell lines, such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells, that report on one or more pattern recognition receptor (PRR), cytokine, or transcription factor activities using SEAP and/or Lucia luciferase, plus a recently launched line of knockout cells that lack major innate immune sensors or signaling proteins;

4-PRR Ligands:

An expanding collection of agonists and control ligands for all the major PRRs, such as Toll-like receptors (TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors (CDSs), including ligands of natural and synthetic origin, all screened and validated in-house;

5-Inhibitors:

A wide selection of functionally validated compounds to directly or indirectly block the principal immune (TLR, NLR, CDS) pathways and related signaling pathways;

6-Antibodies and Recombinant Proteins:

A large variety of biologically validated monoclonal and polyclonal antibodies against major human and murine innate immune targets, as well as isotype families of clinically relevant antibodies, plus a selection of recombinant cytokines;

7-Vaccination:

Pre-clinical grade vaccine adjuvants that comprise alum, emulsions, such as CFA and saponin, and PRR ligands, plus various ovalbumin-derived products.

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