综合电商模板

InvivoGen  pfuselc-hchg1  pFUSE-Lucia-CHIg-hG1

pFUSE-Lucia-CHIg-hG1 is a cloning plasmid that expresses the   constant region of the human IgG1 heavy chain.  It contains a   multiple cloning site upstream of the constant region  to enable cloning   of the heavy chain variable region. This plasmid is  designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSE-Lucia-CHIg-hG1 contain the secreted luciferase (Lucia) gene upstream of the MCS and the CH region to serve as a tag to facilitate the detection and quantification of recombinant antibodies.

产品描述

pFUSE-Lucia-CHIg

pFUSE-Lucia-CHIg  expresses the constant region of the heavy chain (CH) of human, mouse or rabbit immunoglobulin isotypes. They contain a multiple cloning site (MCS) upstream of the constant region to enable the cloning of the variable (VH) region of a given antibody, Fab or scFv.

Moreover, pFUSE-Lucia-CHIg plasmids contain the Lucia luciferase gene upstream of the MCS and the CH region to serve as a tag to facilitate the detection and quantification of recombinant antibodies (direct immunofluorescence). Lucia is a secreted luciferase reporter protein with high protein carrier ability.

As heavy chains cannot be properly secreted in the absence of light chains, detection of Lucia luciferase in the cell supernatants reflects the presence of full-length antibodies.

其他产品线

1-Cell Culture and Transfection Reagents:

Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;

2-Mammalian Expression Vectors:

A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;

3-Reporter Cells:

A growing collection of functionally validated and mycoplasma-free human and murine cell lines, such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells, that report on one or more pattern recognition receptor (PRR), cytokine, or transcription factor activities using SEAP and/or Lucia luciferase, plus a recently launched line of knockout cells that lack major innate immune sensors or signaling proteins;

4-PRR Ligands:

An expanding collection of agonists and control ligands for all the major PRRs, such as Toll-like receptors (TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors (CDSs), including ligands of natural and synthetic origin, all screened and validated in-house;

5-Inhibitors:

A wide selection of functionally validated compounds to directly or indirectly block the principal immune (TLR, NLR, CDS) pathways and related signaling pathways;

6-Antibodies and Recombinant Proteins:

A large variety of biologically validated monoclonal and polyclonal antibodies against major human and murine innate immune targets, as well as isotype families of clinically relevant antibodies, plus a selection of recombinant cytokines;

7-Vaccination:

Pre-clinical grade vaccine adjuvants that comprise alum, emulsions, such as CFA and saponin, and PRR ligands, plus various ovalbumin-derived products.

Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068