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InvivoGen  pvitro2-lucsp  pVITRO2-Lucia/SEAP

Expression plasmids pVITRO2-Lucia/SEAP are developed mainly for in vitro studies. pVITRO2-Lucia/SEAP allows the ubiquitous and constitutive co-expression of two reporter genes:Lucia (secreted luciferase) and SEAP (secreted embryonic alkaline phosphatase). pVITRO2-Lucia/SEAP plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.

pVITRO2-Lucia/SEAP plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-Lucia/SEAP plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.

产品描述

Dual Reporter

InvivoGen provides a collection of dual reporter plasmids, that do not require cell lysis.This plasmids are developped for in vivo or in vitro use and are designed of easy cloning.

Several commonly used reporter proteins are available: GFP (green fluorescent protein) and LacZ (尾-galactosidase) GFP (green fluorescent protein) and SEAP (secreted embryonic alkaline phosphatase) secreted luciferase (Lucia) and SEAP(secreted embryonic alkaline phosphatase)

其他产品线

1-Cell Culture and Transfection Reagents:

Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;

2-Mammalian Expression Vectors:

A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;

3-Reporter Cells:

A growing collection of functionally validated and mycoplasma-free human and murine cell lines, such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells, that report on one or more pattern recognition receptor (PRR), cytokine, or transcription factor activities using SEAP and/or Lucia luciferase, plus a recently launched line of knockout cells that lack major innate immune sensors or signaling proteins;

4-PRR Ligands:

An expanding collection of agonists and control ligands for all the major PRRs, such as Toll-like receptors (TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors (CDSs), including ligands of natural and synthetic origin, all screened and validated in-house;

5-Inhibitors:

A wide selection of functionally validated compounds to directly or indirectly block the principal immune (TLR, NLR, CDS) pathways and related signaling pathways;

6-Antibodies and Recombinant Proteins:

A large variety of biologically validated monoclonal and polyclonal antibodies against major human and murine innate immune targets, as well as isotype families of clinically relevant antibodies, plus a selection of recombinant cytokines;

7-Vaccination:

Pre-clinical grade vaccine adjuvants that comprise alum, emulsions, such as CFA and saponin, and PRR ligands, plus various ovalbumin-derived products.

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