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InvivoGen  rec-lucia  Recombinant Lucia Luciferase Protein

A coelenterazine luciferase encoded by a synthetic gene

Recombinant Lucia Luciferase Protein is a monomeric protein expressed in CHO cells.

The mature protein is composed of 192 amino acids and has an estimated molecular weight of 21 kDa.

Lucia luciferase is a coelenterazine-utilizing luciferase that can be directly measured in the cell culture medium using bioluminescent assays. Recombinant Lucia luciferase is a positive control for luciferase reporter assays.

产品描述

Lucia luciferase

A secreted coelenterazine-utilizing luciferase

InvivoGen’s Lucia luciferase is a completely novel and optimized luciferase with strong bioluminescent activity. It is expressed by a synthetic gene designed on natural secreted luciferase genes from marine copepods.Lucia luciferase generates 1000-fold higher bioluminescent signal compared to the commonly used Firefly and Renilla luciferases. Lucia luciferase is designed for high and prolonged expression in mammalian cells.

Lucia luciferase has several advantages over currently available luciferases and reporter systems:- Lucia luciferase is efficiently secreted into the cell culture supernatant.- Reporter activity is determined fast and easily in real-time without disturbing cells in one simple endpoint assay.- Stable expression of Lucia luciferase makes this reporter system ideal for use in cell based assays and high-throughput applications.The Lucia luciferase reporter system provides an improvement in the sensitivity, reliability and ease of your live-cell reporter gene assays.

Lucia luciferase

其他产品线

1-Cell Culture and Transfection Reagents:

Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;

2-Mammalian Expression Vectors:

A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;

3-Reporter Cells:

A growing collection of functionally validated and mycoplasma-free human and murine cell lines, such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells, that report on one or more pattern recognition receptor (PRR), cytokine, or transcription factor activities using SEAP and/or Lucia luciferase, plus a recently launched line of knockout cells that lack major innate immune sensors or signaling proteins;

4-PRR Ligands:

An expanding collection of agonists and control ligands for all the major PRRs, such as Toll-like receptors (TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors (CDSs), including ligands of natural and synthetic origin, all screened and validated in-house;

5-Inhibitors:

A wide selection of functionally validated compounds to directly or indirectly block the principal immune (TLR, NLR, CDS) pathways and related signaling pathways;

6-Antibodies and Recombinant Proteins:

A large variety of biologically validated monoclonal and polyclonal antibodies against major human and murine innate immune targets, as well as isotype families of clinically relevant antibodies, plus a selection of recombinant cytokines;

7-Vaccination:

Pre-clinical grade vaccine adjuvants that comprise alum, emulsions, such as CFA and saponin, and PRR ligands, plus various ovalbumin-derived products.

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