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InvivoGen fc-hdec1a Fc-hDectin-1a
Bioluminescent conjugate of Streptavidin
The common techniques of ELISA and Western Blot analysiswidely use the Streptavidin-Biotin system for signal amplification. Streptavidin-Lucia features the Lucia luciferase. Streptavidin-Lucia has been optimized for ELISA and can be adapted for use in a variety of assays requiring the detection of biotin and biotinylated proteins.
Streptavidin-Lucia consists of a streptavidin peptide fused to the Lucia luciferase, a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties.
Streptavidin-Lucia uses the advantages of two systems, streptavidin for strength of binding to biotin labeled proteins, together with an optimized luciferase with a strong and stable bioluminescence for high sensitive detection with low background.
Thus, this product is used for the detection of streptavidin-biotin interactions with high sensitivity and accuracy.
The unique long-lasting bioluminescence property of Lucia luciferase allows for ease in reading measurements. After addition of the QUANTI-Luc™ assay reagent to each well of a 96-well plate, results can be obtained in less than a minute using a luminometer without injectors.
产品描述
Soluble PRR Receptors
A secreted coelenterazine-utilizing luciferase
InvivoGen’s Lucia luciferase is a completely novel and optimized luciferase with strong bioluminescent activity. It is expressed by a synthetic gene designed on natural secreted luciferase genes from marine copepods.
Lucia luciferase generates 1000-fold higher bioluminescent signal compared to the commonly used Firefly and Renillaluciferases. Lucia luciferase is designed for high and prolonged expression in mammalian cells.
More info on Luciferase and Lucia luciferase
Lucia luciferase has several advantages over currently available luciferases and reporter systems:
- Lucia luciferase is efficiently secreted into the cell culture supernatant.
- Reporter activity is determined fast and easily in real-time without disturbing cells in one simple endpoint assay.
- Stable expression of Lucia luciferase makes this reporter system ideal for use in cell based assays and high-throughput applications.
The Lucia luciferasereporter system provides an improvement in the sensitivity, reliability and ease of your live-cell reporter gene assays.
Lucia luciferase
Luciferase Assays | |
Bioluminescent conjugate of Streptavidin | |
Reporter Gene System | |
Secreted luciferase detection medium | |
Lucia luciferase Reporter Gene in expression plasmid | |
Positive control for luciferase reporter assays | |
Lucia luciferase Neutralizing antibody - Monoclonal Mouse IgG1 | |
Reporter Cell Lines | |
Reporter cell lines that feature the Lucia luciferase reporter gene | |
Reporter Plasmids | |
Signal Transduction Reporter Plasmids | |
Expression plasmid with a secreted luciferase (Lucia) Tag | |
Dual reporter plasmids - EF-1α promoters - luciferase & SEAP | |
Dual reporter plasmids - Ferritin promoters - Lucia & SEAP | |
Lucia-tagged antibody generation | |
Cloning plasmids with constant regions of the heavy (CH) chain and a secreted luciferase gene | |
Lucia-tagged Fc-Fusion proteins | |
Production of Lucia luciferase-tagged Immunoadhesins | |
其他产品线
1-Cell Culture and Transfection Reagents:
Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;
2-Mammalian Expression Vectors:
A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;
3-Reporter Cells:
A growing collection of functionallyvalidated and mycoplasma-free human and murine cell lines,such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells,that report on one or more pattern recognition receptor (PRR),cytokine, or transcription factor activities using SEAP and/orLucia luciferase, plus a recently launched line of knockout cellsthat lack major innate immune sensors or signaling proteins;
4-PRR Ligands:
An expanding collection of agonists andcontrol ligands for all the major PRRs, such as Toll-like receptors(TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors(CDSs), including ligands of natural and synthetic origin, allscreened and validated in-house;
5-Inhibitors:
A wide selection of functionally validatedcompounds to directly or indirectly block the principal immune(TLR, NLR, CDS) pathways and related signaling pathways;
6-Antibodies and Recombinant Proteins:
A largevariety of biologically validated monoclonal and polyclonalantibodies against major human and murine innate immunetargets, as well as isotype families of clinically relevantantibodies, plus a selection of recombinant cytokines;
7-Vaccination:
Pre-clinical grade vaccine adjuvants thatcomprise alum, emulsions, such as CFA and saponin, and PRRligands, plus various ovalbumin-derived products.
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