InvivoGen  ant-mpt  Plasmocin™ treatment

Plasmocin™ - Mycoplasma Elimination Reagent

Elimination and Prevention of Mycoplasma Contamination in Cell Cultures

Plasmocin™ is used to cure cell lines infected by mycoplasma and related cell wall-less bacteria. Plasmocin™ can also be used as a routine addition in liquid media to prevent mycoplasma and more generally bacterial contamination in small and large animal cell cultures.More info on Mycoplasma eradication

Plasmocin™ is a well-established antimycoplasma reagent. It contains two bactericidal components strongly active against mycoplasmas that allow their elimination in only 2 weeks.

The first component acts on the protein synthesis machinery while the second acts on the DNA replication. These two specific and separate targets are found only in mycoplasmas and many other bacteria and are completely absent in eukaryotic cells.

Plasmocin™ is also active at low concentrations on a broad range of Gram positive and Gram negative bacteria that are otherwise resistant to the mixture of streptomycin and penicillin, and exhibits no toxicity in eukaryotic cells.

Many cell lines infected by mycoplasmas have been successfully treated with Plasmocin™ [1], including primary cancer cell lines [2], virus producing cells [3] and induced pluripotent stem cells [4], without inducing any permanent alterations. Further, in a recent study, a 2 week treatment with Plasmocin was found to effectively cure mycoplasma contamination in human embryonic stem cells without affecting their stemness and pluripotency nor cell viability [5].

Warning: InvivoGen's anti-mycoplasma products are suitable for research purposes only, and not for human or animal care.

Specifications

• Plasmocin Prophylactic (2.5 mg/ml): to prevent mycoplasma contamination, use at 2.5 - 5 µg/ml on a regular basis

• Plasmocin Treatment (25 mg/ml): to eliminate mycoplasmas, use at 25 µg/ml for 2 weeks Treat up to 25 cell lines in T75

Contents:

Plasmocin™ is provided as a cell culture tested, sterile filtered yellow solution at different concentrations:

- 25 mg/ml (Plasmocin™Treatment)

- 2.5 mg/ml (Plasmocin™ Prophylactic)

产品描述

Mycoplasma Elimination & Detection

Mycoplasma contamination represents a significant problem to the culture of mammalian cells used for research. Mycoplasmas can cause disastrous effects on eukaryotic cells as they can alter every cellular parameter leading to unreliable experimental results and potentially unsafe biological products. Mycoplasmas cannot be detected by visual inspection using a normal light microscope and thus, can remain unnoticed in the cell cultures for long periods.

Mycoplasma Elimination

Mycoplasma contamination can be efficiently and rapidly eliminated with the Mycoplasma removal agents, Plasmocin™ or Plasmocure™. They combine two antibiotics that act through different mechanisms and allow mycoplasma eradication in only 2 weeks. More info on Mycoplasma eradication

Mycoplasma Detection

InvivoGen has developed PlasmoTest™, a cell-based assay that provides a simple, rapid and reliable method to detect all mycoplasma species known to infect cell cultures. More info on Mycoplasma detection

Warning: InvivoGen's anti-mycoplasma products are suitable for research purposes only, and not for human or animal care.

Mycoplasma Elimination & Detection

其他产品线

1-Cell Culture and Transfection Reagents:

Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;

2-Mammalian Expression Vectors:

A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;

3-Reporter Cells:

A growing collection of functionally validated and mycoplasma-free human and murine cell lines, such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells, that report on one or more pattern recognition receptor (PRR), cytokine, or transcription factor activities using SEAP and/or Lucia luciferase, plus a recently launched line of knockout cells that lack major innate immune sensors or signaling proteins;

4-PRR Ligands:

An expanding collection of agonists and control ligands for all the major PRRs, such as Toll-like receptors (TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors (CDSs), including ligands of natural and synthetic origin, all screened and validated in-house;

5-Inhibitors:

A wide selection of functionally validated compounds to directly or indirectly block the principal immune (TLR, NLR, CDS) pathways and related signaling pathways;

6-Antibodies and Recombinant Proteins:

A large variety of biologically validated monoclonal and polyclonal antibodies against major human and murine innate immune targets, as well as isotype families of clinically relevant antibodies, plus a selection of recombinant cytokines;

7-Vaccination:

Pre-clinical grade vaccine adjuvants that comprise alum, emulsions, such as CFA and saponin, and PRR ligands, plus various ovalbumin-derived products.

Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068