InvivoGen  rep-pt1  PlasmoTest™ - Mycoplasma Detection Kit

PlasmoTest™ provides a simple, rapid and reliable assay for the visual detection of mycoplasma contamination in cell cultures. This assay is the first to utilize cells to signal the presence of mycoplasma. PlasmoTest™ features two major constituents: the Mycoplasma sensor cells and the HEK-Blue™ Detection medium. The Mycoplasma sensor cells detect the presence of mycoplasmas leading to a color change of the HEK-Blue™ Detection medium. The Mycoplasma sensor cells recognize mycoplasmas through Toll-like Receptor 2 (TLR2), a pathogen recognition receptor. In the presence of mycoplasmas, TLR2 initiates a signaling cascade leading to the activation of NF-kB and other transcription factors. These transcription factors induce the secretion of SEAP (secreted embryonic alkaline phosphatase) in the supernatant which is readily detected by the purple/blue coloration of the HEK-Blue™ Detection medium.

产品描述

PlasmoTest™ - Mycoplasma Detection

Detection of Mycoplasma Contamination in Cell Cultures

PlasmoTest™ provides a simple, rapid and reliable assay for the visual detection of mycoplasma contamination in cell cultures. This assay is the first to utilize cells to signal the presence of mycoplasma. More info on Mycoplasma detection in cell culture

Buy the PlasmoTest™ kit once then reorder only the reagents to perform further assays. The reagents can be purchased individually or together as the PlasmoTest™ Reagent Kit.

其他产品线

1-Cell Culture and Transfection Reagents:

Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;

2-Mammalian Expression Vectors:

A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;

3-Reporter Cells:

A growing collection of functionally validated and mycoplasma-free human and murine cell lines, such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells, that report on one or more pattern recognition receptor (PRR), cytokine, or transcription factor activities using SEAP and/or Lucia luciferase, plus a recently launched line of knockout cells that lack major innate immune sensors or signaling proteins;

4-PRR Ligands:

An expanding collection of agonists and control ligands for all the major PRRs, such as Toll-like receptors (TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors (CDSs), including ligands of natural and synthetic origin, all screened and validated in-house;

5-Inhibitors:

A wide selection of functionally validated compounds to directly or indirectly block the principal immune (TLR, NLR, CDS) pathways and related signaling pathways;

6-Antibodies and Recombinant Proteins:

A large variety of biologically validated monoclonal and polyclonal antibodies against major human and murine innate immune targets, as well as isotype families of clinically relevant antibodies, plus a selection of recombinant cytokines;

7-Vaccination:

Pre-clinical grade vaccine adjuvants that comprise alum, emulsions, such as CFA and saponin, and PRR ligands, plus various ovalbumin-derived products.

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