Sigma-Aldrich  T9935  胰蛋白酶 来源于牛胰腺

产品编号 T9935 | CAS号 9002-07-7 | SIGMA

胰蛋白酶 来源于牛胰腺

Trypsin from bovine pancreas

essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture

产品名称

Components

• 用于组织培养板上贴壁细胞的释放以进行传代。

Caution

• Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca²⁺ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.

Preparation Note

• 来源于新西兰产地的胰脏

Unit Definition

• 1 BTEE 单位 = 320 ATEE 单位

Application

• For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Biochem/physiol Actions

• Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

性质




Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Cell Dissociation, Cell Dissociation Enzymes,
product line	BioReagent
form	essentially salt-free, lyophilized powder
mol wt	mol wt 23.8 kDa
composition	protein, ≥80%
solubility	hydrochloric acid: soluble1 mM
suitability	suitable for cell culture
storage temp.	−20°C




安全信息




符号	GHS07, GHS08
信号词	Danger
危害声明	H315-H319-H334-H335
警示性声明	P261-P305 + P351 + P338-P342 + P311
个人防护装备	dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码 (欧洲)	Xn
风险声明 (欧洲)	36/37/38-42
安全声明 (欧洲)	22-24-26-36/37
RIDADR	NONH for all modes of transport
WGK德国	1
RTECS	YN5075000







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