Roche  11681451001  NBT/BCIP Stock Solution

公司简介

罗氏总部位于瑞士巴塞尔，在制药和诊断领域是世界领先的以研发为基础，以创新为驱动的健康医疗公司之一。作为全球最大的生物技术公司之一，罗氏在抗肿瘤、抗病毒、炎症、代谢和中枢神经系统领域拥有切实有效的差异化药品；同时罗氏也是体外诊断和基于组织的肿瘤诊断的市场领导者，以及糖尿病管理领域的先驱者。罗氏倡导的“个性化医疗”方案旨在提供创新的药品和诊断工具，从而切实改善人类的健康水准、生活质量以及患者的生存期。

在成功收购德国宝灵曼公司（Boehringer Mannheim GmbH.）后，成立了专业服务于科学研究领域用户的罗氏诊断生命科学部（Roche Life Science），凭借半个多世纪在生命科学研究领域积累的经验，罗氏诊断生命科学部已成为在此领域中具重要地位的试剂及系统供应商之一。罗氏诊断生命科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断生命科学部为客户进行量身定制产品，满足客户个性化需求。

产品介绍

General description

• The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.Formulas: NBT: C₄₀H₃₀Cl₂N₁₀O₆, BCIP: C₈H₆NO₄BrClP x C₇H₉NEvaluation: visually

Other Notes

• 仅用于生命科学研究。不可用于诊断。

Preparation Note

• Working solution: Preparation of staining solutionAlways prepare this solution fresh shortly before use. • For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl₂.• For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaClNote: Do not include MgCl₂ in the detection buffer as this may lead to spotty background on the membrane after the detection procedure. Insoluble in water.

Physical form

• Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).Note: The color of the solution can vary from yellowish to light brown.

Application

• • The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot• Northern blot• Western blot^[3]• Colony and plaque lifts• In situ hybridization^[4][2]• and for applications in immunohistochemistry and immunocytochemistry.Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.

性质




Related Categories	Colorimetric, Nucleic Acid Detection Substrates and Reagents, 分子生物学, 核酸检测和杂交
form	solution
mol wt	mol wt 817.7
packaging	pkg of 8 mL
mfr. no.	Roche
shipped in	wet ice
storage temp.	2-8°C







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