Roche 04906837001 PHOSS-RO PhosSTOP™

公司简介

罗氏总部位于瑞士巴塞尔，在制药和诊断领域是世界领先的以研发为基础，以创新为驱动的健康医疗公司之一。作为全球最大的生物技术公司之一，罗氏在抗肿瘤、抗病毒、炎症、代谢和中枢神经系统领域拥有切实有效的差异化药品；同时罗氏也是体外诊断和基于组织的肿瘤诊断的市场领导者，以及糖尿病管理领域的先驱者。罗氏倡导的“个性化医疗”方案旨在提供创新的药品和诊断工具，从而切实改善人类的健康水准、生活质量以及患者的生存期。

在成功收购德国宝灵曼公司（Boehringer Mannheim GmbH.）后，成立了专业服务于科学研究领域用户的罗氏诊断生命科学部（Roche Life Science），凭借半个多世纪在生命科学研究领域积累的经验，罗氏诊断生命科学部已成为在此领域中具重要地位的试剂及系统供应商之一。罗氏诊断生命科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断生命科学部为客户进行量身定制产品，满足客户个性化需求。

产品介绍

Features and Benefits

• Achieve immediate, effective, and convenient inhibition of a broad spectrum of phosphatases across a wide range of sample materials (e.g., mammalian, plant, yeast, and bacteria) with non-toxic PhosSTOP Phosphatase Inhibitor Cocktail Tablets. PhosSTOP is a proprietary blend of phosphatase inhibitors, formulated as a ready-to-use, quick-dissolving, water-soluble tablet.• Comprehensive protection: Inhibit acid and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases.• Safe: Dissolve the non-toxic PhosSTOP Tablets in aqueous solutions and avoid organic solvents (e.g., DMSO).• Suitable for formalin fixation: Maintain phosphorylation status of proteins in histological sections.• Convenient: Eliminate the time-consuming search for just the right phosphatase inhibitor.• Easy to use: Simply drop a quick-dissolving tablet into your buffer.• Stable: Store stock solutions for 1 month at +2 to +8°C, or for at least 6 months at -15 to -25°C. For all-inclusive protein protection, combine PhosSTOP with cOmplete or cOmplete ULTRA Protease Inhibitor Tablets to inhibit both phosphatases and proteases.

General description

• PhosSTOP tablets are a proprietary blend of phosphatase inhibitors, formulated as a ready-to-use tablet which dissolves quickly in aqueous solutions (buffers), including buffers containing formalin (for formalin fixation).

Other Notes

• 仅用于生命科学研究。不可用于诊断。

Preparation Note

• Working concentration: 1 tablet per 10 ml cell extract or bufferWorking solution: Solvent is recommended in distilled water or aqueous buffers (including buffers containing formalin).Dissolve one tablet per 10 ml aqueous buffer. Alternatively, prepare a 10x stock solution in 1 ml dist. water or 100 mM phosphate buffer, pH 7.0. Higher concentrations of stock solutions (e.g., 20x) are possible.Storage conditions (working solution): 2 to 8 °C

Specificity

• Protects proteins against dephosphorylation. Inhibits phosphatase classes such as acid and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases (PTP) in bacterial, mammalian, yeast, and plant extracts.

Application

• PhosSTOP^™ protects protein extracts against dephosphorylation. The tablets inhibit a broad spectrum of phosphatases such as acidic and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B), dual-specificity phosphatases and tyrosine protein phosphatases (PTP) in bacterial, insect, mammalian, yeast, and plant extracts.^[1][3][2] PhosSTOP Tablets are well suited for the purification and detection of phoshorylated proteins.^[4] They are also used to prevent dephosphorylation in formalin-fixed paraffin embedded (FFPE) tissue sections.PhosSTOP tablets can be applied in combination with cOmplete Protease Inhibitor Cocktail Tablets to simultaneously protect proteins against dephosphorylation and proteolytic degradation. Both inhibitor tablets maintain their activity when used together.PhosSTOP inhibitors were evaluated for their effect on protein assays. The inhibition shows almost no influence on BCA or Bradford protein assays.

法律信息

• PhosSTOP is a trademark of Roche

性质




Related Categories	Enzyme Inhibitors, Protease Inhibitors, 生化试剂, 蛋白酶抑制剂Cocktails, 酶、抑制剂和底物
form	tablet
packaging	pkg of 10 tablets (04906845001)
	pkg of 20 tablets (04906837001)
mfr. no.	Roche
solubility	aqueous buffer: soluble
shipped in	wet ice
storage temp.	2-8°C







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